黄芩素通过GPR30信号通路抑制雌激素诱导乳腺癌细胞迁移、黏附和侵袭-药理学专业毕业论文.docxVIP

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黄芩素通过GPR30信号通路抑制雌激素诱导乳腺癌细胞迁移、黏附和侵袭-药理学专业毕业论文.docx

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万方数据万方数据和AKT的磷酸化水平以及CYR61和CTGF基因的mRNA水平。结果表明，黄芩素能够抑制GPR30信号通路的激活以及下游基因的表达。结论：（1）黄芩素能够抑制E2诱导细胞迁移的能力、与基底胶黏附的能力以及侵袭的能力；（2）黄芩素能够抑制GPR30信号通路的激活以及下游相关基因的表达; （3）黄芩素可作为治疗雌激素受体GPR30阳性乳腺癌转移的候选药物。【关键词】黄芩素；雌激素；G蛋白偶联受体30；迁移；黏附；侵袭；乳腺癌 III Baicalein suppresses 17-β-estradiol-induced migration, adhesion and invasion of breast cancer cells via the G protein-coupled receptor 30 signaling pathway Major: Pharmacology Postgraduate: Shang Dandan Supervisor: Prof. Wang Xudong, Prof. Wan lei [Abstract] Objective: The aim of this study was to discuss wheather baicalein can suppresses 17-β-estradiol-induced migration, adhesion and invasion of breast cancer cells via the G protein-coupled receptor 30 signaling pathway. Methods: The following breast cancer cell lines were used as the model system: estrogen receptor (ER)-/GPR30-positive MCF-7 cells and (ERα)-negative/GPR30-positive SK-BR-3 cells. The cell model was induced by E2 or G1, MTT assay was used to examine cell proliferation; Wound-healing assay was applied to study cell migratory ability; Cell adhesion assay study was used to test adhesive ability of cell to the extracellular matrix; Transwell chamber assay coated with matrigel was used to study cell invasion ability; Western blotting assay was performed to observe protein expressions; Real time PCR was used to investigate the mRNA levels of GPR30 -related genes, including CYR61 and CTGF. Results: (1) Baicalein at 5, 10 and 15 μM had no obvious cytotoxic effect on the MCF-7 or SK-BR-3 cells in the presence or absence of E2, and these concentrations were used in following study; (2) E2 stimulation for 24 h significantly increased the migration rate. After treatment with 10 and 15 μM baicalein for 24 h, the migration rate decreased in the MCF-7 or SK-BR-3.These results demonstrated that baicalein suppressed E2-induced cell migration; (3) E2 greatly increased cell-Matrigel adhesion when compared with the control, but the adhesive cells was reduced after treatment with 5, 10, and