慢性高原病骨髓有核红细胞凋亡及Bcl-2表达研究-内科学(血液病学)专业毕业论文.docxVIP

青海大学医学院 青海大学医学院 2010 级攻读临床医学硕士学位研究生毕业论文 PAGE PAGE 10 英文摘要 The Studies on Erythroblasts Apoptosis and Bcl-2 Expression in Chronic Mountain Sickness Postgraduate student：Sun Min-min Abstract Objective Chronic mountain sickness (CMS), characterized by excessive erythrocytosis and hypoxia, is a common disease among people at high altitude. It is caused by complicated reasons. It was confirmed in many studies that excessive proliferation of red blood cells and apoptosis change of hematopoietic cells is associated with its pathophysiology. In recent years, more and more studies suggested apoptosis change is closely related to the pathogenesis of CMS. The Bcl-2 family was important for apoptosis regulation, and it is an important regulatory factor of mitochondria-dependent pathway. The purpose of the study was to observe the expression of Bcl-2 and apoptosis index of erythroblasts in patients with CMS, elucidating the effect of apoptosis and the Bcl-2 family in the occurrence of excessive erythrocytosis. Method The CMS patient was diagnosed by ―Consensus statement on chronic and subacute high altitude diseases ‖[1](2004, Qinghai, Chnia ).There were 16 male CMS patients with (43.40±14.90) years old of average age in the CMS group, and 16 simple fracture patients for orthopedics with (43.37±10.07) years old of average age in the Control group. The bone marrow specimens were collected from posterior superior iliac spine. The BMMNCs were separated and cultured in vitro for collection of erythroblasts. The apoptotic index of erythroblasts was determined by TUNEL technique, the expressions of Bcl-2 mRNA in erythroblasts were measured by RT-PCR. This study compared the apoptotic index and Bcl-2 mRNA expressions between the two groups, and analyzed the relationships among the Bcl-2 levels, apoptotic index and hemoglobin concentration . Results ①The apoptotic index in the CMS group (7.35%±3.74%) was Significantly lower than that in the control group （18.1%±