毕业论文：《葡萄逆境胁迫诱导型启动子的克隆》.docVIP

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毕业论文：《葡萄逆境胁迫诱导型启动子的克隆》.doc

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PAGE 青岛农业大学毕业论文题目: 葡萄逆境胁迫诱导型启动子的克隆姓名: 刘景祎学院: 生命科学学院专业：生物技术班级： 2006级2班学号：指导教师：薛仁镐完成时间： 2010.6.14 2010年6月目录中文摘要···································································································1 Abstract·····································································································2 1．引言···································································································3 2．材料与方法··························································································6 2.1 材料····································································································6 2.2 方法····································································································6 2.2.1引物序列的设计··············································································· 2.2.2 组培培育葡萄幼苗·········································································· 2.2.3 DNA的提取··········································· 2.2.4 PCR扩增········· 2.2.5 凝胶电泳检测···································· 2.2.6 基因片段回收································ 2.2.7 与T载体连接及产物转化··········································· 2.2.8 质粒的提取················································································ 2.3.9 酶切鉴定··························································································· 3．结果与分析···························································································9 3.1 DNA提取鉴定·······················································································9 3.2 PCR扩增结果·······················································································10 3.3 质粒提取鉴定········