三氧化二砷诱导RA滑膜细胞凋亡机制的体外研究-内科学 风湿病专业论文.docxVIP

天津医科大学硕士研究生学位论文Abstract 天津医科大学硕士研究生学位论文 Abstract Objective．To investigate and analyse the possible mechanism of arsenic trioxide (AS203)that induces apoptosis of human fibroblastlike synoviocytes(HFLS)by activating the mitechondria to release Cytochrome c(cyto，then to activate Caspases， 011 the basis of culturing the synoviocytes in vitro,SO intra-ceUular apoptosis may be stimulated．Moreover,it啪regulate the pathway by affecting Bcl-2 mRNA Methods：Primary synoviocytes were cultured by mgans of two-enzymatic digestion． Accroding to the foregone research that the cultured cells were identified HH