两亲性聚合物合成及抗菌性能研究-化学 高分子化学与物理专业论文.docxVIP

兰州大学2010届硕士学位论文 兰州大学2010届硕士学位论文 刘耀斌 两亲性聚合物合成及抗菌性能研究 摘 要 近年来抗菌材料的研究成为国内外重要的前沿课题，目前研究 的抗菌聚合物由于抗菌的广谱性和选择性不理想，因而在具体应用中 (特别是生物材料)受到诸多限制。所以研究合成高抗菌广谱性和选 择性的聚合物具有重要的意义。本课题采用传统自由基聚合和可逆 加成．断裂链转移聚合(Reversible Addition-Fragmentation Chain Transfer Polymerization)技术合成了系列不同亲水／疏水链段比的共 聚物，并测试了其抗菌性能。抑菌圈法和最低抑菌浓度法、浊度法表 明了制备的两种两亲性共聚物均具有良好的抗菌活性，溶血性测试表 明聚合物均没有血细胞毒性。鉴于嵌段共聚物在抗菌方面的研究报道 很少，我们探讨了影响嵌段共聚物抗菌能力的因素。本课题通过传统 活性聚合和RAFT活性／“可控”聚合制各的无规和嵌段共聚物，较 系统的研究了功能化后的两亲性聚合物的抗菌性能，证明了理论上合 成两亲性抗菌共聚物的方案是完全可行的。为当前制备高抗菌性、高 选择性以及广谱性的高聚物合成拓宽了思路，以飨读者。 关键词 高聚物，嵌段共聚物，抗菌材料，RAFT 兰州大学2010届硕士学位论文 兰州大学2010届硕士学位论文 刘耀斌 两亲性聚合物合成及抗菌性能研究 Abstract In recent years，research of antibacterial materials is becoming an important frontier issue at home and abroad．Currently antibacterial property and spectrum of material are not satisfactory,SO they are limited by common applications(especially biological materials)．Therefore，it is of great significance to synthesize broader spectrum of selectivity and higher antibacterial material．In this study,we synthesized hydrophobic／ hydrophilic segments of antimicrobial polymers via the traditional radical polymerization and reversible addition—fragmentation chain transfer polymerization．Zone of Inhibition Testing and Minimum Inhibitory Concentration method，Turbidity method are used to show that both the random copolymer and block copolymer have excellent antibacterial activity and hemolytic experiment shows that they are not toxic to rabbit blood cells．In view of few reports about antimicrobial application of block copolymer,we discussed possible factors affecting antibacterial activity of the block copolymer．This study we synthesized random and block copolymer through traditional polymerization and RAFT controlled ／living”polymerization，it has demonstrated that it is feasible to synthesize highly antibacterial amphiphilic copolymers．We hope readers will obtain useful information of preparation of higher antimicrobial and broader spectrum