超声波技术在猪脱钙骨基质制备中的应用研究-第三军医大学学报.DOCVIP

超声波辅助脱钙技术用于猪脱钙骨基质制备的实验研究的效果评价 任智超，王 欣，刘宝林 ( 200093 上海，上海理工大学医疗器械与食品学院) ［摘要］ 目的 以猪后腿骨干骺端为对象，考察超声波技术在脱钙骨基质制备过程中的作用。方法 6月龄公猪后腿骨清洗切片，分别用传统脱钙制备法和超声波辅助脱钙制备法进行脱钙骨基质的制备，超声波功率：220 W，频率：25 kHz。超声波处理时间：0～6h。以脱钙骨的脱钙率考察超声波对脱钙效果的影响；利用Micro-CT和显微镜观察脱钙骨的大体三维结构；测量其孔隙率和孔径大小；利用物性测试仪测量脱钙骨的压缩模量以表征其力学性能。结果 脱钙骨脱钙率的测量结果显示，与传统脱钙法相比，超声波辅助脱钙制备法仅需6 h就可使脱钙率达到100%，脱钙时间可缩短45.5%；且制备的脱钙骨具有良好的孔隙率和互相连通的孔径结构，孔隙率达到85%以上，孔径大小为(348.18±64.86) μm，与传统制备方法无显著差异(P＞0.05)；并具有良好的力学性能，在脱除90%以上的钙后脱钙骨的压缩模量仍可达到(6.42±0.83) N/mm2。结论 超声波辅助脱钙技术可以有效提高脱钙骨基质的制备效率，且制备效果良好，为超声波技术在脱钙骨制备领域的应用提供了试验依据。 ［关键词］ 猪、干骺端、超声波、脱钙骨 ［中图法分类号］ R318 [文献标志码] A The Effect of Ultrasonic on the Preparation of Demineralized Bone Matrix from Metaphysis of pig Ren Zhichao, Wang Xin, Liu Baolin (School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai Province, 200093, China) ［Abstract］ Objective The effect of ultrasonic on the preparation of demineralized bone matrix from the discarded metaphysis of pig was investigated. Methods The decalcification rate was measured. The porosity and pore size of demineralized bone matrix was investigated by Micro-CT. And the mechanical properties of demineralized bone matrix was shown as modulus of compressibility. Results The decalcification rate indicated that the decalcification process can be finished within 6 hours, when using the ultrasonic method, which could reduce 45.5% preparation time of the traditional method. And the demineralized bone matrix has similar porosity(85%) and pores connection to the traditional method (P＞0.05).The pore size was (348.18±64.86)μm. After losing 90% calcium, the modulus of compressibility can still maintain as (6.42±0.83)N/mm2, which means an acceptable mechanical properties. Conclusion The preparation rate could be improved significantly by the ultrasonic technique. It also provided basis for the utilization of ultrasonic technique. ［Key words］ Pig; Metaphysis; Ultrasonic;