兰茵凤扬化浊解毒方对溃疡性结肠炎免疫机制的实验研究-中医内科学专业论文.docxVIP

』嬲㈣目 』嬲㈣ 目 录 中文摘要 0 0 1 英文摘要 4 研究论文兰茵凤扬化浊解毒方对大鼠溃疡性结肠炎免疫机制 的实验研究 引言 ······ ·······”eOO．OOO 000 7 第一部分兰茵风扬化浊解毒方对溃疡性结肠炎大鼠一般状态、 结肠组织形态学的影响 前言···日{J舌”一“”“””一”“一””“”””””“““””””””“””。······ ··· ······ ······ ········· ······ OOO”一“”””99 材料与方法 9 结果000 000 OOOlOO 012 附图OOO 60“．．””一””““”“”“”””““”“”“一”“”””“”“·14 附表 000 00(0 20 讨论··············································································2l 参考文献 ．．． 22 第二部分兰茵凤扬化浊解毒方对溃疡性结肠炎大鼠T细胞亚群影响 前言······················································”·”········”·”“·23刖吾”一”““””一””“””一““””””””“”“一””一”””””””“。Z3 材料与方法 23 结果·········000 OQO·”””““””24 附图OOO OOO 26 附表 27 讨论······························“······．．·一”··一····一”“一”一．．””””28 参考文献 30 第三部分兰茵风扬化浊解毒方对溃疡性结肠炎大鼠细胞因子 IL．8、IL．10的影响 前言···O月O声”‘ OO················································““””一”“”””·3”“””“”““””””“¨“””””““”””““””一”“”””。j 1l 材料与方法 3 1 结果··········································“····“”””·OO 00”．．”32 附图O 0 000 0 O 0 33 附表 34 讨论··················································· 讨论··················································· ············· ········35 参考文献 ； ··37 结论····················································································39 综述···················· ··············-············ ·········· ··················40 致谢···························000 0“······················0 000············49 个人简历 50 中文摘要兰茵凤扬化浊解毒方对溃疡性结肠炎 中文摘要 兰茵凤扬化浊解毒方对溃疡性结肠炎 免疫机制的实验研究 摘 要 目的：溃疡性结肠炎(UC)是一种病因尚未完全阐明的慢性炎症性肠 病，主要临床表现为腹泻、粘液脓血便、腹痛和里急后重。导师据多年 临床经验总结，制定协定方兰茵凤扬化浊解毒方，其临床疗效肯定，副 作用少。本课题通过兰茵风扬化浊解毒方对UC大鼠模型的干预，特别 是通过观察其对T细胞亚群及细胞因子(IL．8、IL．13)的影响，深入揭示兰 茵风扬化浊解毒方治疗UC的作用机制，为中医药治疗UC提供一定的理 论依据和实验基础。 方法：实验选取健康清洁级晰star大鼠60只。将大鼠随机分为5组： 空白对照组(Nomal control group)、模型组(Model group)、柳氮磺胺毗啶 组(SASP)、中药大剂量组(Large soup group)、中药小剂量组(Small soup group)。除空白对照组正常喂养外，其余四组大鼠均采用2，4，6．三硝基 苯磺酸(TNSS)／乙醇联合造模法诱导大鼠溃疡性结肠炎动物模型。造模 后第3天分别给予兰茵风扬化浊解毒方大剂量、小剂量和柳氮磺胺吡啶进 行灌胃治疗，观察其疗效并探讨其作用机制。本实验通过三个方面研究 探讨：一，观察兰茵凤扬化浊解毒方对UC大鼠一般情况、结肠组织病理 形态学的影响；二，观察兰茵风扬化浊解毒方对UC大鼠T淋巴细胞亚群 的影响；三，观察兰茵风扬化浊解毒