莱克多巴胺胶体金试纸条的研制及生产线设计-食品工程专业论文.docxVIP

|ffllfI|l|fIjf|Iflilf|fI|mf{lflfffIffIffff|Iff埘{Y1 |ffllfI|l|fIjf|Iflilf|fI|mf{lflfffIffIffff|Iff埘 {Y1 942982 摘要 摘要 本文采用柠檬酸三钠还原法制备胶体金，通过调节柠檬酸三钠溶液的加入 量制备出3种不同粒径的胶体金，最终选择2011111左右粒径的胶体金标记单抗蛋 白。对标记条件进行了优化，最适标记蛋白添加量为9．6舭，最适标记pH为 8．0，标记后选择l％BSA作稳定剂。经过试验选择心S仃om6613为金标结合垫， Milliporel35为层析膜，PB75为样品垫，抗原包被浓度为2m咖L，包被二抗为 20倍稀释度。试纸条灵敏度可达5n咖L，在小于200n劝nL浓度范围内，与类 似物克伦特罗、沙丁胺醇、多巴酚丁胺、肾上腺素等均无明显交叉反应，在室 温条件下可以保存3个月以上。 本文中的设计主要是对于日产量为1000条莱克多巴胺残留检测试纸条生产 线的设计。根据试纸条的研制试验，确定工艺流程，在此基础上完成了试纸条 生产过程中的物料衡算和主要生产设备选型，最后绘制了工艺流程、车间平面 布置等图。本设计依据有关规定，满足GMP规范，在设计时考虑了以下几点： 1)设计了缓冲间、更衣室、气闸室等保证了空气的洁净度；2)人流物流分开 避免了交叉感染。 关键词：莱克多巴胺；胶体金；免疫层析；生产线 II AbstractAbstract Abstract Abstract In tllis paper，nle colloidal gold w雒prepared by tIle tri—sodium citrate reduction memod．Three l【inds of di虢rent sizes colloidal gold were prepared by adding di妇Ferem amoums of tri-sodium citrate．’11le colloidal goId of 20Iml w嬲chose．The key experiment cond“ions of prep撕ng gold·nlonocloIlal antibody were optimized． The suitable虹ble concen垃ation was 9．6“咖L甜ld me optimal pH was 8．0． According to tests results，we chose Alllstrom6613，Milliporel35，PB75 aS conjugate pad，s锄ple pad，11i仃ocellulose filter membrane to make ColloidaJ gIold teSt strip． Coating concen仃ation of antigen waS 2m咖L and coating secondary antibodyⅥ倍S dilmed to l／20．11le Sensitivi锣of teSt s臼jps could reach 5ng／mL．When me concen衄ation of an2Llogue w∞less t11an 200n咖L，“had n0 si星皿ificant cross reaction谢t11 clenbllterol，salbutamol，dobutamine aIld epinep¨ne．The teSt strip could be stored at room temperature more thaIl 3 montIlS． n is皿marily desiglled for colloidal gold strips production l沁of one thousalld daily ou印ut ．The products process now w嬲 dete皿ined according to 也e deVelopmem of teSt strips．On t11is basis，the calcmatioIls of material balaIlce in the production pmcess aIld equipment selection were conlpleted．FiIlally，me now chan aIld noo印laIl were drew．The desigIl was accorded t0 relev趾t regulations aIld met 目录目 目录 目 录 第1章前言 l 1．1莱克多巴胺概述 ．．1 1．1．1莱克多巴胺的结构和性质 1 1