刺五加原花青素超声波提取稳定性工艺.docVIP

大连工业大学2013届本科生毕业论文 PAGE 11 - 摘 要 刺五加是我国东北地区典型的药用植物，有很高的营养价值。本实验以酿酒剩余的刺五加残渣中刺五加籽为原料，提取原花青素，增加刺五加的附加价值。 本实验利用超声波辅助提取，从刺五加籽中提取原花青素，优化提取条件，并讨论光照、温度、常见金属离子对原花青素稳定性的影响。以原花青素的提取率为指标，通过单因素实验确立影响原花青素提取量的适宜条件，并通过正交实验确定提取的最佳条件。实验结果表明，原花青素提取的最佳工艺条件为：溶剂乙醇浓度为55%，刺五加籽和乙醇的料液比为1:50，超声时间为25min，超声功率为480W，提取率最高，可以达到0.792%。原花青素对光较为敏感。当温度低于60℃时，稳定性较好；当温度超过60℃时，其稳定性较差。所以原花青素应在低温避光的条件下储存、加工。对于常见金属离子，Cu2+对原花青素的影响较大，K+、Ca2+、Mg2+对原花青素影响较小，因此在原花青素提取、精制和加工过程中应尽量避免Cu2+的使用 关键词：刺五加；原花青素；超声波；提取；稳定性 Abstract Acanthopanax is a typical medicinal plant in the northeast of China,with a high nutritional value.This experiment extracted proanthocyanidins from Acanthopanax seeds to improve the Acanthopanax value.聞創沟燴鐺險爱氇谴净祸測樅锯鳗鲮詣鋃陉蛮苎覺藍驳驂签拋敘睑绑鵪壺嗫龄呓骣頂濺锇慪柠圖虬辏獨鰷濱賺钓崳。 Ultrasonic wave assisted extraction from Acanthopanax seeds and the influence of light,temperature and common metallic ion were studied in this paper.With the extraction ratio as index，the optimal extraction conditions were determined by the single factor and orthogonal experiment. In the ultrasonic extraction experiments,the results showed that the reaction conditions were concentration of alcohol 55%, solid-liquid ratio 1:35, ultrasonic time 25 min, ultrasonic power 480W,and extraction ratio of proanthocyanidins was 0.792%.Proanthocyanidins were sensitive to light.And its stability was bad when the temperature is over 60℃.So proanthocyanidins shuold be stored under the condition of low temperature and darkness.Proanthocyanidins had good stability for K+,Ca2+,Mg2+,and bad for Cu2+.So it should avoid using Cu2+ in the process of extracting and refining proanthocyanidins.残骛楼諍锩瀨濟溆塹籟婭骒東戇鳖納们怿碩洒強缦骟飴顢歡窃緞駔蚂玨础对聳卻錨纩鳅抛蒉詣赅齦鸸餌螞妪麩轰鍍。 Key Words: Acanthopanax; proanthocyanidins; ultrasonic; extraction; stability酽锕极額閉镇桧猪訣锥顧荭钯詢鳕驄粪讳鱸况閫硯浈颡閿审詔頃緯贾钟費怜齪删费龙觯諞餛鸬挣紐攄线幀鲑泽谶绗。 目 录 TOC \o 1-4 \h \z \u 摘 要 I彈贸摄尔霁毙攬砖卤庑诒尔肤亿鳔简闷鼋缔鋃耧泞蹤頓鍥義锥柽鳗铟夺髅搅联黨莢蠷抛務槍渖鐋颠聶鹭铹釹诫诎響。 Abstract II謀荞抟箧飆鐸怼类蒋薔點鉍杂篓鳐驱數硯侖葒屜懣勻雏鉚預齒贡缢颔臉悭榇龟伤确妫閽缮该賴爐满鐵薺硷蓝骤蚂釗。 第一章 文献综述 1 1.1 刺五加研究进展 1 1.1.1 刺五加