利用选择回交导入系定位和聚合水稻抗旱QTL-作物遗传育种专业论文.docxVIP

AbstractDrought Abstract Drought is one of the most iIIlportant factors limiting the production in rice(Oryza sativa L)．It is themost simple and effectivemethodstoimprovedroughtto】crance(DT)ofricevaritiesbydiscovering and applying DT QTLs from germplasm．The new strategy of molecular breeding to combine QTL discovery witII crop breeding Call be conducted by screening the target trait of introgressed lines 0Ls) derived from large scale backcross and genotyping ILs．Furtherly,DT QTb can be pyramided by intercross DT ILs with favorable DT QTLs from diff雠nt donors assisted by marker selection to develop DT rice varieties． In this study,C418．an elite japonica restorer of Northern China,was used recurrent parent to cross and backcross with C71 and Zaoxian 14(donors)respectively,from which the random BC2F2 populations were developed．The bulks of 2 BC．2F2 populations wel-e imposed critical selections for drought tolerance fOX)under站veTe drought stress，from which a total of 16 introgression lines(ⅡJs) survived the stress．The progeny of the DT BC21％ⅡJ were repeatedly evaluated for DT and genotyped with molecular IIIR．rkel％for DT QTL detection．Pyramiding populations were developed by making ClOSses between 4 of DT lLs and imposed DT selections in F2 under the severe drought condition，from which 2 sets of selected DT pyramiding lines(VLs)were repeatedly phenotyped for DT and genotyped with molecular markers for QrL analysis．ne main results summarized follows： 1．The progeny test of 2 sets of DT ILs we∞conducted for DT under drought and irrigation conditions in Hainan and B喇ing．T]Rwe existed some variation．s between populations,experiment locations and water treatments．In sunmlm,no significant differences were observed between recunent parent and the ILs in DT with the grain yield as the DT index．However,some lLs in the progeny performed much better DT and yield than the recurrent parenL Among the grain yield components， panicles per plant and grams per panicle were closely correla