类芽孢杆菌F53生防活性和絮凝活性研究-微生物学专业论文.docxVIP

类芽孢杆菌F53生防活性和絮凝活性研究40d，表明这种拈抗菌在土壤中的定殖能力强。接种茄子黄萎病菌对F53的定殖 类芽孢杆菌F53生防活性和絮凝活性研究 40d，表明这种拈抗菌在土壤中的定殖能力强。接种茄子黄萎病菌对F53的定殖 有较大影响，特别是接种黄萎病菌3d后接种F53和同时接种黄萎病菌和F53的 情况下，F53的定殖能力下降，提示F53对病害的预防效果更优。 此外，类芽孢杆菌F53能产生大量的胞外多糖，对高岭土的絮凝活性可达 72．3％，因而F53还是～株具有较高絮凝活性的菌株。本试验对胞外多糖进行了 提取和纯化，通过颜色反应、薄层层析及红外光谱分析等实验，结果表明F53胞 外多糖的基本结构单元中主要为葡萄糖。 关键词：类芽孢杆菌，固体制剂，生物防治，胞外多糖，絮凝 II 类芽孢杆菌F53生防活性和絮凝活性研究A 类芽孢杆菌F53生防活性和絮凝活性研究 A Study on Biocontrol and Flocculation Activity of Paenibacillus elgii F53 Abstract Paenibacillus elgii F53 was obtained from eggplant rhizosphere,which has proved with better inlfibiting activity to the pathogenic fungus verticillium dahliae．In order to obtain the F53 solid preparation for field used，a series work was developed， involving wim optimizing solid fermentation experiment．testing the safety for germination ofvegetables，promoting growth effects and controlling infection and its colonizating ability in the plant roots．and SO on．111e strain F53 could produce extracellular polysaccharide and had higher flocculating activity．Thus，the component and flocculating activity of its extraeellular polysaccharide had also been analysed． The solid substrates of F53 were primarily selected from 5 kinds of single substrate and combination solid substrates by an otthogonal test．The results showed that in the best direction of solid substrate，weight ratio of bran，grain pieces and cornmeal WaS 2：1：1．The effects of ferment temperature，ratio of solid materials and water,pH and inoculum concentration on endospore yield and inhibiting compounds were studied．Accumulation ofantifimgal compound was directly correlative wim the quantities of endospomlation in the solid fermentation．The solid ferment conditions were optimized as follows：temperature 35C，ratio of solid substrate and water 1：1．4。 pH 7．0，10％inoculum． The effects of solid fermentation preparation on the seed germination of tomato， cucumber,eggplant and rape were carried ouL It Was indicated that F53 resulted in different germination percent