桦褐孔菌子实体与菌丝体三萜化合物提取及活性比较研究.PDFVIP

※基础研究 食品科学 2012, Vol. 33, No. 15 161 桦褐孔菌子实体与菌丝体三萜化合物提取及 活性比较研究 朱春玉，郑方亮，邵丽杰，冯华炜，郭立雄，刘宏生* ( 辽宁大学生命科学院，辽宁省生物大分子计算模拟与信息处理工程研究中心， 辽宁省动物资源与疫病防治重点实验室，辽宁 沈阳 110036) 摘 要：为探索桦褐孔菌菌丝体与子实体在药用价值上是否存在差别，采用有机溶剂冷浸、超声回流法提取桦褐 孔菌菌丝体及子实体的三萜化合物，通过薄层层析和红外光谱分析，确定两种来源的三萜化合物是相同或近似的同 系物。四甲基偶氮唑盐比色法(MTT)和杯碟法测定两种来源三萜化合物对癌细胞和细菌的抑制作用，菌丝体三萜化 合物对人胃癌细胞株MGC-803 的最高抑制率可达到38.2 7% 。结果证实人工培养的桦褐孔菌菌丝体同样具有子实体 的药用价值，为人工培养桦褐孔菌菌丝体提供了依据。 关键词：桦褐孔菌；三萜化合物；抗肿瘤；抑菌活性 Extraction and Bioactivity of Triterpenoids from Fruit Bodies and Mycelia of Inonotus obliquus ZHU Chun-yu ，ZHENG Fang-liang ，SHAO Li-j ie ，FENG Hua-wei ，GUO Li-xiong ，LIU Hong-sheng* (Key Laboratory of Animal Resource and Epidemic Disease Prevention, Research Center for Computer Simulating and Information Processing of Bio-Macromolecules of Liaoning, College of Life Science, Liaoning University, Shenyang 110036, China) Abstract：In order to explore the difference in medicinal value of cultured mycelia and fruit bodies of Inonotus obliquus, organic solvent cold soaking and ultrasonic reflux were adopted to extract triterpenoids from fruit bodies and mycelia of Inonotus obliquus. The triterpenoids extracted from both sources were determined to be the same or similar homologues by thin layer chromatography (TLC) and Fourier tran sform infrared (FT-IR) spectral analysis. Both triterpenoid extracts could inhibit the growth of cancer cells and bacteria as determined by tetrazolium salt assay (MTT) and cylinder plate method. The highest inhibitory rate of triterpenoids to human gastric cancer cell line MGC-803 reached up to 38.27%. Therefore cultured mycelia of Inonotus obliquus have the same medicinal value as fruit bodies