药学与生物工程学院-入选分论坛文章__学术论文.docVIP

药学与生物工程分论坛 目录 TOC \o 1-1 \p \u 基于制备环境的家蚕丝胶蛋白的抗菌及细胞毒性作用……………………………………….……… 3 子宫颈癌疫苗的研究现状 ……………………………………………………………………...……….. 11 CTL表位改造的研究进展 ………………………………………………………………………… 15 重组人胰岛素在毕赤酵母中的表达及纯化 ………………………………………………………...….. 22 鸡蛋中单增李斯特菌的检测 ………………………………………………………………………...….. 28 反向离子电渗透原理无创血糖检测研究进展 ……………………………………………………..….. 34 基于分子对接对二氢吡唑，二氢吡咯衍生物类Eg5抑制剂的3D-QSAR研究 …………………...….. 41 Three dimensional holographic vector of atomic interaction field (3D-HoVAIF) for quantitative structure–activity relationship (QSAR) of vascular endothelial growth factor receptor tyrosine kinase inhibitors ………………………………………………………………………………………… 53 Predicting the activity of peptides based on amino acid information …………………... 75 基于银纳米颗粒的局域表面等离子共振光纤传感器 …………………………………………………. 87 Estimation of Vascular Elasticity Based on DFT Analysis of Radial Pulse Waveform …. 95 基于制备环境的家蚕丝胶蛋白的抗菌及细胞毒性作用 摘 要: 水煮脱胶法从茧壳和茧衣中提取丝胶蛋白（Jk、Jy），将部分茧壳丝胶酶解得小分子的丝胶蛋白肽（Jkmj）。研究这三种丝胶蛋白抗金黄色葡萄球菌和大肠杆菌作用，采用细菌生长抑制定量法测试梯度浓度下各丝胶的抑菌率；采用MTT细胞毒性实验测试梯度浓度下各丝胶对L929细胞的相对增殖率,确定毒性等级。结果显示：不同环境中制得的丝胶蛋白均有良好的抗菌性能,对金黄色葡萄球菌的抑制作用：JkmjJkJy，对大肠杆菌抑制作用：JyJkmjJk；且丝胶的浓度对其抑菌性也有明显影响。Jk的细胞毒性评分随其浓度的增大从0级增至1级，高浓度的Jk对细胞生长有略微影响；而各浓度的Jy和Jkmj的RGR毒性评分均为0级，有促细胞生长作用，且随浓度的增大，促生长作用越明显。Jy 和Jkmj比Jk细胞相容性好。 关键词: 丝胶蛋白；制备环境；抗菌性；细胞毒性 Antibacterial properties and cytotoxicity of sericin proteins based on different preparation environment QinLuo, Yaoqin Zhang (College of Pharmacy and Bioengineering，Chongqing University of Technology, Chongqing 400054) Abstract: Sericin proteins were extracted from cocoon shell (Jk) and cocoon outer floss (Jy) by water boiled degumming method. And the sericin protein peptide (Jkmj) was prepared from cocoon shell by neutral protease. Their antibacterial properties under concentration gradient against Staphylococcus aurous and Escherichia coli were compared using the bacterial inhibition rate by bacterial growth inhibition quantitative test. MTT method was carried out to estimate the cytotoxicity of each sericin protein at concentration gradient on L929 c