微透析法研究七叶胆苷抗大鼠脑缺血损伤作用.docVIP

微透析法研究七叶胆昔抗大鼠脑缺血损伤作用 (1.黑龙江中医药大学 黑龙江 哈尔滨150040) (2.哈尔滨师范大学黑龙江哈尔滨150025) (3.药用植物研究所云南分所药理中心 云南 景洪666100) 摘 要 目的研究七叶胆昔对局灶性脑缺血脑透析液中谷氨酸(glutamic acid, Glu)和gamma;-氨基丁酸(gamma aminobutyric acid, GABA)神经递 质水平的影响，探讨七叶胆昔对缺血性脑组织损伤的保护作用。方法SD大鼠 分为假手术组(生理盐水1 mLkg-lmiddot;d-l)^模型组(生理盐水ImL kg-lmiddot;d-l) 七叶胆昔组(20rngmiddot;kg?lmiddot;d-l)和尼莫地平组 (2.5 mgmiddot;kg-lmiddot;d-l)o各组每天腹腔注射给药，连续给药7天后， 复制大鼠大脑中动脉栓塞模型，进行微透析取样。所得样品经衍生化后，于高效 液相色谱仪中测试，比较透析液中氨基酸神经递质的变化。梗死部分经235■氯 化三苯基四氮^(2,3,5-Triphenyltetrazolium chloride, TTC)染色，计算梗死率。结 果七叶胆昔组大鼠脑透析液中谷氨酸和gamma;-氨基丁酸水平均明显降低， 脑梗死率明显降低。结论七叶胆昔可以通过减少脑缺血后谷氨酸和gamma;- 氨基丁酸的释放，降低脑组织梗死率，起到抗脑缺血的作用。 关键词 七叶胆昔；脑缺血；微透析；谷氨酸；gamma;-氨基丁酸。 R619 B 1003-5028 (2015) 6-0651-02 The Study about Gype no side agai nst Cerebral Ischemic In jury by Microdialysis in Rat Lei Shuangyuanl, Wu Sulil, Qi Yonghual, Li Guang3, Liu Guoliangl, Zhang Ningl*, Geng Fang2* (1.Heilongjiang University of Traditional Chinese Medicine, Harbin 150040; 2.Harbin Normal Universit, Harbin 150025; 3.Yunnan Branch Institute of Medicinal Plant, Chinese Academy of Medical Sciences」nghong 666100 ) Abstract] Objective To study the effects of Gypenoside on Glu and GABA neurotransmitter levels in focal cerebral ischemia cerebral rats brain micro-dialysates. Method Sprague-Dawley rats were randomly divided into sham operation group, model group, Gype no side group (20mgmiddot;kg-1middot;d-l) and Nimodipine group (2.5 mgmiddot;kg-lmiddot;d?l). The rats in each group were administrated by intraperitoneal injection for 7 days. The middle cerebral occlusion model was established on the 7th day, and the brain micro-dialysates were collected. The brain micro?dialysates were derivatised and analyzed by High performanee liquid chromatography to compare the changes of amino acid neurotransmitters in micro-dialysates. The ischemic areas were calculated by TTC stain。Results The Glu and GABA levels in brain dialysates of Gypenoside group were reduced, the cerebral infarction perce ntage were decreased to