离子色谱在食品分析中的应用研究-环境化学专业论文.docxVIP

万方数据 万方数据 摘要 离子色谱在食品分析中的应用变得越来越广，本实验研究利用离子色谱的方法 对可食用样品中不同的目标物进行分离分析，取得了良好的实验结果： 一、利用聚乙二醇对自制的Fe3O4纳米粒子进行表面改性，并以芦丁为模板分 子，利用悬浮聚合法制备了芦丁-磁性分子印迹聚合物。实验证明该磁性分子印迹聚 合物对样品中的芦丁的吸附过程为单分子层吸附。建立了离子色谱法对样品中芦丁 的分析测定方法，PA20色谱柱、NaOH淋洗液，方法简单，灵敏度高，精密度好适 用于样品中芦丁含量的测定。 二、建立了一种超声提取-离子色谱法对水产品中氟含量进行分析的方法，采用 AS-15HC作为分离柱，以KOH溶液做淋洗液进行梯度洗脱。方法具有灵敏度高、精 密度好、操作简便、样品基质干扰小、准确度高等优点。建立一种离子色谱-电导检 测器检测饲料样品中六价铬的方法，采用AG19-AS11色谱柱进行分离；样品经磷酸 缓冲液、碱性提取液及MgCl2·6H2O固体处理后，基质对六价铬测定过程中的干扰较 小。方法消除了前处理过程中引入的Cl-、磷酸根离子等杂质的干扰，准确性高。 三、建立了一种离子色谱法对饮料制品中天然糖的测定方法，得出葡萄酒样品 中均含有葡萄糖、果糖和阿拉伯糖三种天然糖，少量葡萄酒样品中含有L-山梨糖。 饮料样品中主要含有大量的葡萄糖、果糖和蔗糖三种糖，部分饮料样品中含有少量 木糖。该方法适用于饮料制品中八种常见天然糖的含量测定。通过离子色谱-质谱的 方法对样品中单糖及部分二糖进行分析，可对样品中的单糖或二糖进行定性测定。 通过选择[M+Na]+作为糖类物质分析过程中的母离子，并通过柱后衍生的方式使母 离子响应值变得稳定。方法可以消除五碳糖和六碳糖之间的相互干扰，对样品中糖 类物质的准确分析具有指导意义。 关键词：离子色谱、芦丁、阴离子、天然糖、离子色谱-质谱 I Abstract The application of Ion Chromatography (IC) in food analysis is becoming more and more widely. In this research, different target of edible sample were analyzed by IC, and we obtained good experimental result. By suspension polymerization, we prepared the Magnetic Molecularly Imprinted Polymer of Rutin’s on the surface of Fe3O4 nanoparticles which surface was modified by Polyethylene glycol. The adsorption process for Rutin of this polymer was Monolayer adsorption. We developed a method of analysis the Rutin by IC, which was eluented by NaOH, and using the PA20 column for separation. The method is simplify, and high sensitivity, good precision, is suitable for the determination of rutin content in the samples. Using NaOH gradient eluent, we developed a method for Fluoride analysis by IC, and the sample matrix were separated on AS11-HC column. This method has much advantages, such as high sensitivity, good precision, convenient operation, small sample matrix interference and high accuracy and so on. We also developed a method for Cr (VI) analysis by IC. At the pretreatment process, the sample was extracted by Phosphate Buffer, Alkaline extract and MgCl2?6H2O, and the interference of C