高分子聚合物微流控细胞芯片的改性研究-第三军医大学学报.doc

编号 201109231 刊用形式 论著 高分子聚合物微流控细胞芯片的改性研究 赖梦君1，张惠静1,2，张莉2，曹文轩2，管潇2（1重庆大学化学化工学院，重庆 400030；2第三军医大学医学检验系药物分析与分析化学教研室，重庆 400038） [摘要] 目的 探讨两种不同硅烷化试剂对高分子聚合物材料PDMS芯片的改性效果和改性后芯片在细胞捕获/筛选的初步应用。方法 接触角实验考察不同硅烷化试剂处理的PDMS样本亲水性，筛选硅烷化试剂及其浓度，以芯片上的非特异性细胞捕获实验验证筛选结果，并连接抗体构建功能化芯片，进行细胞捕获实验的初步研究。结果 两种硅烷化试剂均对PDMS表面具有改性作用，其中在体积浓度为2%GPTMS处理后的芯片表面测得的接触角值最小，芯片对细胞非特异性吸附为5.3%，固载抗体后芯片对细胞的净捕获率为53.96%。结论 本研究筛选出PDMS硅烷化反的试剂为GPTMS，最佳浓度为2%，此条件下材料的亲水性良好，对细胞的非特异性吸附低，可用于功能化细胞捕获芯片的构建。 [关键词] PDMS；表面修饰；硅烷化；微流控芯片 [中图法分类号] R318.08：R392-33：R730.45 Study of Surface Modification on Polymer Microfluidic Cell Chip Lai Mengjun1，Zhang Huijing1,2，Zhang Li2，Cao Wenxuan2，Guan Xiao2（1Chemistry and Chemical Engineering College；Chongqing University, Chongqing , 400030, P.R.China；2Department of Analytical Chemistry，College of Laboratory Medicine，Third Military Medical University，Chongqing，400038，P.R.China） [Abstract] Objective To study the Surface modification effect of PDMS by two different silylation reagents and a Initial application of the Microfluidic chip. Methods Hydrophilicity of samples treated by silylation reagents was measured by contact angle, screening silane reagent and its concentration. Result was verificationed by non-specific cell capture experiments. Then Immobilized antibody to get preliminary experiments for cell capture studies. Results In this study, two silylation reagents was used to reduce the non-specific adsorption. It shows that 2%(volume concentration) GPTMS could get a smallest contact angle and non-specific adsorption of cells reduced to 5.3%, capture rate of cells was 53.96%. Conclution An effective silylation reagent was found and screened the best concentration for surface modification of PDMS chip. In this condition, PDMS was Hydrophilic and had a non-specific adsorption of sells. This will ues for functioned cell capture chips. [Key worlds] Poly dimethylsiloxane(PDMS)，surface modification，silylation，Microfluidic chip Supported by the Key