结直肠癌中miR106b增强肿瘤细胞放疗抵抗作用机制的初步分析.docVIP

结■肠的 miR-106b增强W删胞放I?抵fit作顽 制删蚪析 2018-03-20 01:26:12中国现代医生2018年1期 杨丽君万晓晨 ［ ］目的探讨结直肠癌中miR-106b增强肿瘤细胞放疗抵抗作用机制。方法釆用RT-PCR 技术检测结直肠癌细胞株当川的miR-106b的表达，分析英对放疗抵抗的影响。结果检测SW620 细胞放疗敏感性，miFM06b过表达Z后SW620细胞的生存分数显著高于对照组(P0.05)；检测 miR-106b抑制表达的SW480细胞放疗敏感性，miR?106b稳定干扰之后SW480细胞的生存分数要 显著低丁对照组(PvO.05)：平板克隆实验结果表明，SW620/plVTHM/miR-106b细胞增殖能力显 著强于SW620/plVTHM细胞(P0.05)o结论miR/06b可以强化细胞抵抗射线导致的DNA损伤, 提高细胞修复DNA的能力，最终强化结直肠癌细胞放疗抵抗作用。 ［关键词］结直肠癌;miR-106b；肿瘤细胞；放疗抵抗;机制 ［中图分类号］R735.34 ［文献标识码］A ［文章编号］1673-9701 (2018) 01-0029-03 Preliminary analysis on the mechanism of miR-106b enhancing the radioresistanee of tumor cells in colorectal cancer YANG Lijun WAN Xiaochen Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, China ［Abstract］ Objective To investigate the mechanism of miR-106b enhancing the radioresistanee of tumor cells in colorectal cancer. Methods The expression of miR-106b in colorectal cancer cell lines was detected by RT-PCR? Its impact on radioresistanee was analyzed. Results The radiosensitivity of SW620 cells was detected. The survival score of SW620 cells after miR-106b overexpression was significantly higher than that in the control group (P0.05) ； the radiosensitivity of miR-106b to inhibit the expression of SW480 cells was detected, and the survival score of SW480 cells after miR-106b stable interfere nee was significa ntly lower than that in the control group (P0.05) ； the results of plate cloning experiments showed that the cell proliferation of SW620/plVTHM/miR-106b cells was significantly stronger than that of SW620/plVTHM cells (P0.05 )? Conclusion miR-106b can enhance the cell resistance to radiation-induced DNA damage, improve the ability of cells repair!ng DNA. and ultimately enhance the radioresistanee of colorectal cancer cells? ［Key words］ Colorectal cancer； miR-106b； Tumor cells； Radioresistance； Mechanism 結直肠癌发生发展同遗传因素以及环境因素等存在密切联系，统计结果显示，结直肠癌临床发 病率近年来呈现出明显升高的发展趋势。当前情况下，结直肠癌患者的治疗主要是手术治疗联合辅助 放疗口］。有效放疗可以杀死癌症细胞，同吋延长细胞周期，改善于?术治疗效果。不过部分患者的放疗 敏感性比较低，远期效果不够理想，因此放疗抵抗作用