不同蚕蛹油中脂肪酸的性状及组分分析-食品科学.pdfVIP

148 2011, Vol. 32, No. 04 食品科学 ※分析检测 1 1 1 , 2 1 1 , 潘文娟 ，方婷婷 ，廖爱美 ，张海祥 ，魏兆军 * (1.合肥工业大学生物与食品工程学院，安徽 合肥 230009 ；2.合肥师范学院生命科学系，安徽 合肥 230061) 研究不同蚕蛹油的脂肪酸种类和相对含量。采用气相色谱 - 质谱方法，对 KOH/CH3OH 酯交换法甲酯化 4 种不同的蚕蛹油进行定量分析。结果表明：柞蚕蛹、蓖麻蚕蛹、家蚕蛹及剿丝后家蚕蛹油的酸价、皂化值和过 氧化值存在显著差别；4 种蛹油的脂肪酸成分的种类都以棕榈酸、硬脂酸、油酸、亚油酸、α- 亚麻酸为主，但 含量存在显著区别；4 种蚕蛹油中，柞蚕蛹油的不饱和脂肪酸相对含量是最高的，达 77.29% ；蓖麻蚕蛹油的α- 亚麻酸相对含量最高，为 50.52% ；缫丝后家蚕蛹油不饱和脂肪酸的相对含量比缫丝前降低。 蚕蛹油；脂肪酸组分；气相色谱 - 质谱 Character and Fatty Acid Composition of Silkworm Pupal Oil 1 1 1,2 1 1, PAN Wen-juan ，FANG Ting-ting ，LIAO Ai-mei ，ZHANG Hai-xiang ，WEI Zhao-jun * (1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China； 2. Department of Life Science, Hefei Normal University, Hefei 230061, China) Abstract ：In order to determine the types and relative contents of fatty acids in 4 kinds of silkworm pupal oil, oil transesterification was carried out with KOH/CH3OH, followed by gas chromatography-mass spectrometric analysis. Results indicated that acid values, saponification values and peroxidation values differed significantly among the 4 kinds of pupal oil. All the four tested materials contained the following major components: linolenic acid (LNA), palmitic acid, stearic acid, oleic acid and α-linolenic acid, which exhibited different relative contents. Among them, the relative content of unsaturated fatty acids in oak silkworm pupal oil was the highest, which was up to 77.29% and castor silkworm pupal oil contained the most α-linolenic acid, with a relative content of 50.52%. Desilked pupal oil contained mor