木二糖和木三糖的分离及其用于双歧杆菌的体外培养-林产化学与工业.pdf

25 1 Vo.l 25No. 1 2005 3 Chem istry and Industry of ForestProducts M ar. 2005 * 张军华, 徐 勇, 勇 强, 卜晓莉, 余世袁 (南京林业大学 化学工程学院, 江苏 南京 210037) : 采用凝胶过滤层 技术获得了高纯度木二糖和木三糖,并研究了它们在体外对青春 双歧杆菌的增殖效果以聚丙烯酰胺凝胶( Bio-GelP- 2)为层 介质,采用快速蛋白液相层 (FPLC)系统为平台, 以脱气高纯水洗脱,制备分离了木二糖和木三糖,并分别以它们为碳源, Z ANG J 研究了体外增殖培养青春双歧杆菌的生长历程结果表明, 体外培养 48 h后, 木糖木二糖和 木三糖分别将青春双歧杆菌菌体浓度从 0. 10 g/L增殖至0. 160. 33和 0. 47 g/L,木三糖对青春双歧杆菌的增 殖效果最好, 木二糖次之,木糖的增殖效果不明显木二糖和木三糖在体外培养青春双歧杆菌 48 h后, 能分 别将体系的 p 值从 7. 0降到 4. 40和 4. 42 : 低聚木糖;木二糖; 木三糖;凝胶过滤层 ;双歧杆菌 : TQ281; Q53 : A : 0253- 2417( 2005) 01- 0015- 04 PREPARATIVE SEPARATION OF XYLOBIOSE AND XYLOTRIOSE AND T EIR IN VITRO APPLICATION IN FERMENTATION BY BIFIDOBACTERIA Z ANG Junhua, XU Yong, YONG Q iang, BU Xiaol,i YU Shiyuan (College of Chem icalEng ineering, Nanj ing F orestry University, N anj ing 210037, Ch ina) A bstrac t: Xylobiose and xylotriosewere prepared from xylooligosaccharides by gel permeation chromatography, and their in v itro cultivation properties byB if i obacterium a olescentis were investigated. Xylobiose and xylotriose were separated on polyacrylam ide gel BioGel P2 by FPLC system w ith degased highpurified water as eluant, and their biological characteristics toward bifidobacteriawere studied. The results showed that the celldryweights ofB. a oles centis were0. 16, 0. 33 and 0. 47 g/L, respectively, when xylose, xylobiose and xylotriosewere used as carbon souces after 48 h cultivation in v itro. Xylotriose had superior effect than xylobiose and xylose