南京农业大学考研复习酶学.ppt

核酶 ribozyme 1982年T. Cech等人发现四膜虫的rRNA前体能在完全没有蛋白质的情况下进行自我加工，发现RNA有催化活性 1983年S.Altman等研究RNaseP（20%蛋白质和80%RNA），发现RNaseP中的RNA可催化E. coli tRNA的前体加工 Ribozymes （核酶） Satisfy several enzymatic criteria: substrate specificity, enhance reaction rate, and emerge from reaction unchanged; Several known ribozymes: RNase P: catalyzes cleavage of precursor tRNA molecules into mature tRNAs; Group I, II introns （内含子）: catalyze their own splicing (cleaving and ligating); Peptidyltransferase (肽酰基转移酶): catalyzes peptidyl transfer reaction (----23SrRNA) What is the difference between an enzyme and a pr