蛋白质的表达-PPT精选文档.pptVIP

An Example of protein investigation;Expression of protein;人工合成尿素;蛋白质的生物合成; In vitro ： in vitro protein synthesis system Prokaryotic Expression System - E. coli, B. subtilis In vivo yeast Eukaryotic Expression System insect cells mammalian cells;香蕉与奶牛;Ⅱ. Prokaryotic Expression System ;1.基因表达在原核生物与真核生物中的差别？;2.真核基因在原核系统表达的困难及其解决方法;3.大肠杆菌表达载体的组件;Prokaryotic Expression System;复制起始点;选择性基因;多克隆位点 ;3.1启动子;P tac = 3 P trp = 11 P lac;