中人氟安对裸鼠腹腔化疗安全性的实验研究.docxVIP

中人氟安对裸鼠腹腔化疗安全性的实验研究 1?新疆医科大学附属肿瘤医院胃肠外科乌鲁木齐830011； 2.新曬医科大学附 属肿瘤医院肺内一科 乌鲁木齐830011 【摘 要】 目的：探讨不同剂量氟尿“密碇植入剂（中人氟安，Sinofuan）治疗 裸鼠恶性腹腔积液的有效性及安全性。方法：釆用人胃癌MKN-45细胞株建立裸 鼠腹膜移植瘤模型，32只成瘤裸鼠随机分为4组，即赋型剂对照组、氟尿卩密噪 植入剂低剂量组（2mg/只）、中剂量组（4mg/只）和高剂量组（6mg/只）。严密 观察各组裸鼠的一般状态、体重变化和牛存期，测量腹水体积，显微镜下计数每 毫升腹水中的肿瘤细胞数。结果「与对照组相比，氟尿卩密旋植入剂低剂量组（2mg/ 只）状态良好，体重增长缓慢，全身症状出现较晚，生存期延长（26天vs 19 天，P = 0.020）；中剂量组于给药第2天死亡一只，高剂量组于给药后6天内全 部死亡。氟尿卩密噪植入剂低剂量组的腹水体积少于对照组（（5.96#61617;1.26） ml vs （10.96#61617;1.21） ml）, P =0.000）；每毫升腹水中的肿瘤细胞数也少于 对照组（（2.75#61617;0.57） times;108bull;ml-l vs （4.21#61617;0.75） times;108bull;ml-l, P二0.034）。结论：低剂量（2mg/只）的氟尿喃旋植入剂 对于裸鼠MKN?45腹膜移植瘤的形成具有良好的抑制作用，可以延长腹膜移植瘤 裸鼠的生存期，且安全性好。裸鼠对中剂量（4mg/R）和高剂量（6mg/只）的 氟尿卩密噪植入剂耐受性差。因此，氟尿喃旋植入剂用于裸鼠腹腔化疗的安全剂量 推荐给予低剂量即2mg/R0 【关键词】 氟尿卩密噪植入剂/中人氟安；胃癌；裸鼠；腹水 [Abstract] Objective To investigate the effect of sustained?release fluorouracil （Sinofuan） injected in MKN?45 ascitic tumor of nude mice.Methods 32 nude mice peritoneal transplanted with MKN-45 cells were randomly divided into 4 groups： control group, low-dose （2mg/nude mouse）, medium-dose （4mg/nude mouse） and high-dose （6mg/nude mouse） sustai ned? release fluorouracil groups.The gen era I state of the nude mice, body mass changes and survival was observed.Ascites volume was measured.The number of tumor cells per milliliter of ascites was counted.Results Compared with the control group, the general state of nude mice from low-dose (2mg/nude mouse) fluorouracil injection group were better with slower weight gain, delayed systemic symptoms and prolonged survival (median survival： 26 days vs 17 days, P lt;0.05)，one nude mouse from medium-dose group died on the second day after intraperitoneal chemotherapy, all nude mice from medium-dose group died within 6 days of the intraperitoneal chemotherapy.Ascites volume and ascetic tumor cells per milliliter of the low-dose sustained?release fluorouracil group were less than the control group[ (5.96#61617;1.26) ml vs (10.96#61617;1.21) ml], P=0.000； (2.75#61617;0.57) times;1