Elsevier期刊投稿英文模板(Animal-Feed-Science-and-Technology).docVIP

PAGE 1 Rumen lysine escape, rumen fermentation and productivity of early lactation dairy cows fed free lysine P.H. Robinsona,*, E.J. DePetersa, I. Shinzatob, H. Satob aDepartment of Animal Science, University of California, Davis, CA, 95616, USA, and Atlantic Dairy Forage Institute, Fredericton Junction, bAjinomoto Co., Inc., 5-8 Kyobashi I-Chome, Chuo-Ku, Tokyo 104, Japan * Corresponding author. Tel: 530-754-7565; Fax: 530-752-0175; EM: phrobinson@ucdavis.edu Submitted to Animal Feed Science and Technology in June 2005 Revised and re-submitted in August 2005. Revised and re-submitted in September 2005. Abstract The primary objective was to quantitate forestomach escape of lysine fed to cows in a free form. However since it was expected that a large proportion of the lysine would be degraded in the rumen, other objectives were to determine if lysine impacted ruminal fermentation as well as determine effects on performance of the cows. Four multiparous Holstein cows, fitted with large diameter rumen cannulae between 6 and 8 weeks prior to their projected calving date, were assigned in a 4 x 4 Latin square design experiment between 2 and 4 weeks post-partum. All cows were fed the same total mixed ration (TMR) and treatment differences were achieved by manually incorporating L-lysine HCl into each cow’s individually weighed allocation of TMR at the time of feeding to deliver 0, 1, 2 or 3 g of L-lysine from L-lysine HCl/kg of dry matter (DM) intake, although actually delivered lysine values were about 16% higher. As expected, average rumen free lysine concentrations increased linearly (P = 0.05) due to increased feeding levels of lysine. Rumen pH, N and volatile fatty acid concentrations, as well as other organic components of rumen ingesta, including those of isolated rumen bacteria, were unaffected by lysine feeding. Intake of DM, neutral detergent fibre and crude protein were not influenced by increased feeding of L-lysine, as were production of milk and