压力性尿失禁治疗用干细胞吊带的构建及其机械.docVIP

UBM编号201207004 刊用形式 论著 复合bMSCs体外构建组织工程化吊带治疗 编号201207004 刊用形式 论著 王颖楠1，范雪梅2，赵 敏3，徐惠成[基金项目] 国家自然科学基金[通信作者] 徐惠成，电话：（023E-mail: xhuicheng@(400038重庆，第三军医大学西南医院妇产科1；610083成都，成都军区总医院妇产科2；315040浙江 宁波，解放军第113医院妇产 [基金项目] 国家自然科学基金 [通信作者] 徐惠成，电话：（023E-mail: xhuicheng@ [摘要] 目的 探讨脱细胞膀胱基质（urinary bladder matrix，UBM）复合大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，bMSCs）构建组织工程化吊带治疗压力性尿失禁的可行性。 方法 采用密度梯度离心法结合贴壁培养法体外分离、培养和扩增bMSCs，流式细胞术检测bMSCs免疫表型，将第3代bMSCs与UBM复合培养，7 d后通过光镜和电镜观察细胞在材料上的生长情况，进一步应用万能力学测试仪检测所构建吊带的最大负荷、杨氏模量、伸展性等机械特性，另以UBM负载大鼠膀胱平滑肌细胞株（smooth muscle cells，SMC）构建SMC吊带、单纯UBM及聚丙烯材料行效果比较。 结果 本实验分离培养的bMSCs呈长梭形，折光性好，增殖旺盛，具有成体干细胞特性，细胞表面标志物CD29阳性，CD45和CD31阴性。UBM去细胞彻底，复合bMSCs或SMC均可获得生长良好的细胞。机械性状检测表明，与SMC吊带及聚丙烯材料相比，单纯UBM 和UBM复合bMSCs吊带的最大负荷、杨氏模量下降，伸展性提高（P0.01）。 结论 成功分离、培养了大鼠bMSCs，并鉴定了其干性。构建了干细胞吊带，与SMC吊带及聚丙烯材料相比，其伸展性良好。 [关键词] 骨髓间充质干细胞；脱细胞膀胱基质；形态学特性；机械特性 [中图法分类号] R318.16；R694+.54 [文献标识码] A Preliminary study on the construction of tissue-engineered sling with bMSCs and UBM in vitro for treating stress urinary incontinence Wang Yingnan1，Fan Xuemei2，Zhao Min3，Xu Huicheng1（1Department of Obstetrics and Gynaecology，Southwest Hospital，Third Military Medical University，Chongqing，400038；2Department of Obstetrics and Gynecology,General Hospital of chengdu Military Command,Chengdu，Sichuan Province，610083；3Department of Obstetrics and Gynecology, No．113 Hospital of PLA，Ningbo，Zejiang Province, 315040, China） [Abstract] Objective To study the possibility of tissue-engineered sling constructed by using bMSCs and UBM for therapy the stress urinary incontinence(SUI). Methods The bMSCs were isolated, cultured and amplified by combining density gradient centrifugation with adhering method.. The amplified bMSCs were identified by FACS. The UBM loaded by 3rd generation of bMSCs for 7d. The morphological characteristics of cultured bMSCs were observed by using