遗传工程菌Fhhh降解精对苯二甲酸与mnp基因表达-环境科学学报.pdf

22 1 Vol. 22, o. 1 2002 1 ACTA SCIE TIAE CIRCUMSTA TIAE Jan. ,2002 :2002 : X172 :A Fhhh mnp 1 1 1 1 1 2 3 程树培 , 陈 亮, 严 俊 , 郝春博 ,李维新, 顾继东,程 攻, 陈兰 3 明 (1 , 210093; 2 ; 3) 2+ : Fhhh (PC) , Mn H O pH 4. pH , 32 . pH PC 2 2 3 , Fhhh 1.Fhhh PC 1111%; mnp (MnP) PC 1522% . 4 , mnp . MnP (r r 0. 05(4) , r r 0. 01(4) ) . . : ; ; ; ; ; Degradation of purified terephthalic acid and expression of mnp gene for GEM Fhhh 1 1 1 1 1 2 3 CHE G Shupei , CHE Liang , YA Jun , HAO Chunbo , LI Weixin , GU Jidong , CHE G Gong , CHE anming3 ( 1The ational KeyLaboratory of Pollution Control and ResourceReuse, anjingUniversity, anjing 210093; 2. Department of Ecology and Biodiversity, Laboratory of EcologicalToxicology, Hong KongUniversity; 3. Department of LifeSciences and Tech niques, anjingUniversity) Abstract:The levels of the specific degradation rate(SDR) forthegeneticallyengineeredmicroorganism ( GEM) Fhhh from interkingdompro toplastfusion and the parental strain Phanerochaete chrysosp orium (PC) in degradation of purified terephthalic acid (PTA) were affected by 2+ ( H ) C H O ,H O ,Mn , and pH value.Except pH value,theother 3factors affecting theSDR levels had a sameorderforboth strains. 4 2 4 4 6 2 2 The pH value in theorderwas at thefirst site for Fhhh, and that for PC at the third site.At the optimal levels,th