朊蛋白在阿尔茨海默病和正常小鼠视网膜组织上的表达分析.pdfVIP

摘要 3.实验组与对照组小鼠视网膜组织中内参因子 β-actin 均表达较稳定，证实 本实验所用 RT-PCR 和 Western Blot 技术可靠，无明显操作误差； 4. RT-PCR 结果显示：阿尔茨海默病小鼠视网膜组织中 PrPc 基因表达水平较 正常小鼠视网膜组织明显增强，差异均有显著的统计学差异 （P0.05 ）；Western blot 结果显示：阿尔茨海默病小鼠视网膜组织中 PrPc 蛋白表达水平较正常小鼠视 网膜组织明显增强，差异均有显著的统计学差异 （P0.05 ）。 结论： 1.阿尔茨海默病小鼠视网膜和正常小鼠视网膜组织中均存在 PrPc 的表达， 且阿尔茨海默病小鼠视网膜的 PrPc 基因和蛋白表达强度均明显强于正常视网膜 组织。2.PrPc 表达主要定位于视网膜内外丛状层、光感细胞层及神经节细胞层上， PrPc 亚细胞定位于细胞膜上。3.阿尔茨海默病组小鼠视盘盘周神经上皮层厚度较 正常组小鼠明显变薄。4. PrPc 与阿尔茨海默病视网膜病变的发生存在相关性。 关键词：细胞型朊蛋白；阿尔茨海默病；视网膜组织；C57小鼠 III Abstract ABSTRACT Objectives : c Observing the cellular prion protein (PrP ) expression on Alzheimers disease (AD) and normal mouse retinal. Meanwhile, Analysis of PrPc and AD mouse retinal tissue change, To provide the experimental basis for studying the pathogenesis of PrPc in further research on Alzheimers disease retinal degeneration , also to provide some research directions for the early diagnosis and early treatment of Alzheimers disease . Methods: Select the size of the 6-month double transgenic (APP / PS1) Alzheimers disease in mouse C57 30 as the experimental group, 6-month-old size 30 normal healthy C57 mouse as a control group, male and female. The mouse were sacrificed after overdose of anesthesia, exposing the eye, separating the conjunctiva, cut the optic nerve of the eye removed, under a dissecting microscopic quickly remove the retina, the retina into the EP tubes, frozen in liquid nitrogen. Firstly, differences in immunohistochemical staining were observed HE AD mouse and normal mouse on the organizational structure of the retina, immune