中药复方抗纤抑癌方和复方鳖甲软肝片不同采血时间点药物血.docVIP

高等学校博士学科点专项科研基金资助课题，课题编号：20060026010 中药复方抗纤抑癌方和复方鳖甲软肝片不同采血时间点药物血清对HepG2肝癌细胞增殖活性的影响# 赵志敏，叶永安＊，杨先照，甘大楠，马贺成，刘方 北京中医药大学东直门医院，北京（100700） E-mail：kilorair@ 摘要 目的 研究人来源不同采血时间点中药药物血清对HepG2人肝癌细胞增殖活性的影响。方法 HepG2人肝癌细胞体外培养，健康志愿者3名，服用中药复方抗纤抑癌方（KX）和中成药复方鳖甲软肝片（BJ）后不同时间点收集血清，采用MTT法检测各组药物血清对细胞增殖的影响。结果 1、1小时、2小时与MEM对照组比较，各实验组药物血清对HepG2人肝癌细胞具有明显的增殖抑制作用（P＜0.01）；KX1小时20%浓度抑制效果优于KX2小时同浓度（P＜0.01）；KX1小时5%浓度与BJ1小时同浓度有差异（P＜0.05）；BJ2小时20%、10%浓度均较KX2小时抑制作用强（P＜0.05）。2、增加0.5小时抽血时间点，KX 0.5小时优于KX 2小时（P＜0.05）。3、与空白血清组比较，KX0.5小时40%浓度时抑制效果明显（P＜0.05）。结论 两种药物血清对HepG2人肝癌细胞均有增殖抑制作用；在同一采血时间点和不同时间点上，两种药物血清的抑制效果比较均存在差异。 关键词 药物血清，采血时间点，HepG2细胞 Influence of Kangxian yiai decoction and Fufang Biejia Ruangan Tablets on HepG2 cells on different blood sampling spots Zhimin Zhao, Yongan Ye, Xianzhao Yang, Danan Gan, Hecheng Ma, Fang Liu (Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing PRC 100700) Abstract Objective: To explore the influence of different kinds of human-derived chinese medicine sera on proliferation of HepG2 cells on different blood sampling spots. Methods: HepG2 cells were cultured in vitro. The sera of 3 volunteers were collected before taking medicine as the control group, twice after taken medicine(Kangxian yiai decoction and Fufang Biejia Ruangan Tablets) 1 hour later and 2 hours again as the test group, MTT colorimetric assay was used to observe the influence on proliferation of HepG2 cells after incubate with different kinds of drug’s containing serum. Results: 1 It showed that The inhibited proliferation in test groups were better than that of control group（P＜0.01）. The inhibited proliferation of the concentration 20% of KX were better after 1hour than that of 2hours, and the difference had statistical significance. Compared with 5% KX group on the 1-hour blood sampling spot, the inhibited proliferation of the concentration 5% of BJ were better, and the difference had statistical significance(P＜0.05).While on the 2-hour blood sampling spot, the inhibited prolifera