西洛他唑抑制颈总动脉球囊损伤后大鼠血凝素样氧化低密度脂蛋白受体-1表达.docVIP

西洛他唑抑制颈总动脉球囊损伤后大鼠血凝素样氧化低密度脂蛋白受体-1表达 苏华科，宋耀明，郭文昀（第三军医大学新桥医院心血管内科，全军心血管病研究所，重庆 400037） 摘 要：目的 观察大鼠颈总动脉球囊损伤后血凝素样氧化低密度脂蛋白受体-1（LOX-1）的表达及西洛他唑对其表达的影响，探索西洛他唑抑制内膜增生的机制。方法 24只健康雄性SD大鼠随机分成假手术组、模型组、西洛他唑组（n=8）。观察球囊损伤后内膜增生情况并计算内膜中膜面积比值，检测血清MDA含量、RT-PCR法和免疫组化法分别检测各组球囊损伤后大鼠颈总动脉血管壁组织中LOX-1 mRNA和蛋白的表达，数据进行统计学分析。结果 西洛他唑组内膜/中膜面积比值（IA/MA）、LOX-1 mRNA及蛋白表达水平、血清MDA含量显著低于模型组，差异有统计学意义（p0.05）。结论 西洛他唑可显著抑制内膜增生，抑制LOX-1表达及其介导的氧化应激可能是西洛他唑抑制内膜增生的机制之一。 关键词：血凝素样氧化低密度脂蛋白受体-1；西洛他唑；球囊损伤；再狭窄 中图法分类号：R543.5 文献标识码：A Effects of cilostazol on expression of lectin like oxidized low density lipoprotein receptor-1 after balloon injury SU Hua-ke, SONG Yao-ming，GUO Wen-yun. Institute of Cardiovascular Disease of People’s Liberation Army, Xinqiao Hospital, Third Military Medical University, Chongqing , Abstract: objective To explore the expression of lectin-like oxidized low-density lipoprotein receptor-1(LOX-1) after balloon arterial injury in a rat model and modulating the effect of cilostazol on the gene and protein expression of LOX-1.Methods twenty-four male SPF SD rats selected were randomly divided into three groups: shan-operation group(n=8); model group; cilostazol group.except shan-operation group,all Left common carotid artery of rats were injuried by a balloon catheter.The expression of LOX-1mRNA and protein in neointimal hyperplasia after balloon arterial injury in a rat model were examined by RT-PCR and immunohistochemistry respectively. the contents of MDA in blood serum was examined by TBA. Results There was expression of LOX-1mRNA and protein in neointimal hyperplasia after balloon arterial injury in a rat model except shan-operation group.The expression level of LOX-1 was significantly lower in the cilostazol group than that in model group (p0.05), the contents of MDA in blood serum was also significantly lower in the cilostazol group than that in model group (p0.05).Conclusion LOX-1 is highly expressed in neointimal hyperplasia after balloon arterial inju