穿心莲内酯抗金黄色葡萄球菌生物膜作用及其与庆大霉素的协同作用.docVIP

穿心莲内酯抗金黄色葡萄球菌生物膜作用及其与庆大霉素的协同作用 周 薇，沈 犁，张燕，张佳星，姜俊如，华子瑜（400014 重庆。重庆医科大学附属儿童医院新生儿科,儿童发育疾病研究教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地） [摘要] 目的 研究穿心莲内酯（Andrographolide, Andro)抗金黄色葡萄球菌（Staphylococcus aureus，S.aureus）生物膜作用及其与庆大霉素(Gentamicin, GEN)的协同作用。方法 选择金黄色葡萄球菌ATCC25923以及两个临床菌株CY001与CY002进行研究。比浊法测定Andro（浓度100μg/mL）对S.aureus各菌株生长的影响；建立S.aureus生物膜体外模型，分为对照组、Andro干预组、GEN干预组以及Andro与GEN联合干预组，结晶紫染色后，酶标仪测量OD600 nm值间接观察各组生物膜的形成能力；扫描电镜下直接观察各组生物膜的结构。 结果 100μg/mL Andro作用下S.aureus各菌株生长曲线与对照组相比无显著性差异（P＞0.05）；除CY002由于不形成生物膜，各组间两两比较均无明显差异外（P＞0.05），CY001与ATCC25923在各干预条件下生物膜均较对照组减少（P＜0.001），100μg/mL Andro对生物膜的抑制作用强于50μg/mL GEN（P值分别为0.001，0.003），两者联用时的抗生物膜作用明显增强（P＜0.05）。扫描电镜下形态观察提示各干预条件对S.aureus生物膜均有抑制作用，以Andro与GEN联用的抑制作用最为明显。 结论 Andro对S.aureus无直接杀菌作用，但对S.aureus生物膜具有良好抑制作用，与GEN联合应用，具有协同抗生物膜作用。 [关键词] 生物膜；金黄色葡萄球菌；穿心莲内酯；庆大霉素 Effect of andrographolide against Staphylococcus aureus biofilm and its synergistic activity with gentamicin Zhou Wei, Shen Li, Zhang Yan, Zhang Jiaxing, Jiang Junru, Hua Ziyu (Department of Neonatology,Key Laboratory of Child Development and Disorders of the Ministry of Education, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, the Children’s Hospital, Chongqing Medical University, 400014, Chongqing, China) [abstract] objective To investigate the effects of andrographolide (Andro) against Staphylococcus aureus (S. aureus) biofilms and its synergistic activity in combination with gentamicin (GEN). Methods Turbidimetric method was used to detect the effect of Andro (100 μg/ml) on S. aureus growth curve. S. aureus ATCC25923 and two clinical strains CY001 and CY002 were used for biofilm formation in vitro, dividing into a control group, an Andro intervention group, a GEN intervention group and an Andro combined with GEN intervention group. A microplate reader was used to detect the OD600nm values and indirectly observe the biofilm formation ability of each group. Sc