AEAPS与葡聚糖共修饰超顺磁纳米颗粒的制备及性质.docVIP

AEAPS与葡聚糖共修饰超顺磁纳米颗粒的制备及性质 邓均1基金项目： 创伤、烧伤与复合伤国家重点实验室开放课题（批准号：SKLKF200809）作者简介： 邓均（1974-），男（汉族），重庆，博士。电话：（023E-mail:djtmmu@? 基金项目： 创伤、烧伤与复合伤国家重点实验室开放课题（批准号：SKLKF200809） 作者简介： 邓均（1974-），男（汉族），重庆，博士。电话：（023E-mail:djtmmu@ ?通讯作者：郑峻松，男，（1971-），研究领域为纳米材料在肿瘤早期靶向诊断的应用。电话：（023E-mail: zhengjunsong@。 （第三军医大学：1医学检验系临床检验学教研室，重庆400038，2学员旅七队，重庆400038） 摘要 目的 探讨以AEAPS及葡聚糖共修饰超顺磁纳米颗粒（superparamagnetic nanoparticles，SPION）的制备方法, 并检测其物理和磁学性质。方法 共沉淀一步法制备葡聚糖包被的四氧化三铁纳米颗粒，然后在通氮、60℃、强力机械搅拌的条件下加入AEAPS，得到AEAPS与葡聚糖共修饰的SPION。采用透射电镜测量其大小及分布，用振动样品磁强计检测磁化率等参数，用普鲁士染色的方法判断AEAPS/葡聚糖-SPION是否与抗体连接。结果 所得样品核心为四氧化三铁晶体，核心粒径不超过20nm，包被葡聚糖后整体颗粒直径不超过25nm，质量饱和磁场强度为67.36488 emu/g Fe，AEAPS/葡聚糖-SPION与抗体结合，可对特异性抗原产生抗原-抗体结合，普鲁士染色结果为阳性。结论 制备的样品具有粒径小，分散性好，超顺磁性等优点，能与单克隆抗体有效结合。 【关键词】超顺磁性氧化铁；AEAPS；纳米粒子；抗体偶联 Preparation and performance test for AEAPS/Dextran-modified SPION DENG Jun, HE Juan, ZHENG Jun-song?, JIA yan-hui, HUANG Hui, FANG Li-chang, LI Yang, Jiang Li-li, LI Ping (1Department of Clinical Laboratory Sciences, College of Medical Laboratory, Tird Military Medical University, chongqing 400038, China, 2Seventh Band, Student Brigade, Tird Military Medical University, chongqing 400038, China ) Abstract: Objective To explore the method of preparation of AEAPS-Dextran-modified SPION ( superparamagnetic iron oxide nanoparticles) and test the physical and magnetic properties of the nanoparticles．Methods Dextran-modified SPION was obtained by “one step” co-precipitation method. Then, AEAPS was added to the resultant Dextran-SPION under the condition of N2 saturation and high-speed mechanical stirring at 60℃. The size and magnetic susceptibility of the nanoparticles were measured by transmission electron microscope and Vibrating Sample Magnetometer, respectively. Results The AEAPS-Dextran-modified SPION nanoparticles had core-shell diameter less than 25nm with magnetic core of less than 20nm in diameter.The mass saturation magnetic intensity is 65.82319emu/g Fe. Antibody conjugated with AEAP