GLP-1受体激动剂艾塞那肽对肿瘤细胞增殖,迁移的影响.docVIP

GLP-1受体激动剂艾塞那肽对肿瘤细胞增殖迁移的影响 张 瑞，刘羞菲，王 慧，王小翠，童 强，郑宏庭（400037重庆，第三军医大学新桥医院内分泌科） [摘要]目的 通过体外细胞实验观察GLP-1受体激动剂艾塞那肽对乳腺癌、结肠癌、胰腺癌三种肿瘤细胞增殖迁移的影响。方法 选取乳腺癌MDA-MB-231细胞、结肠癌HCT116细胞、胰腺癌HS766T细胞三种肿瘤细胞作为实验研究对象，每种细胞分为对照组、二甲双胍组、艾塞那肽组，利用CCK-8分别检测三种肿瘤细胞各个组别72小时后的增殖能力；利用Transwell小室法观察12小时后三种肿瘤细胞各个组别的迁移能力。结果 艾塞那肽组与对照组相比， MDA-MB-231细胞的增殖受到显著抑制（P0.01），HCT116细胞的增殖受到抑制（P0.05），而胰腺癌细胞HS766T细胞增殖无影响（P0.05）。Transwell体外细胞迁移结果显示，艾塞那肽组显著抑制MDA-MB-231细胞迁移（18.10±5.82，P0.01），抑制HCT116细胞迁移（21.20±5.35，P0.05），但是艾塞那肽组却显著增加HS766T细胞迁移（165.40±21.66，P0.01）。结论 GLP-1受体激动剂艾塞那肽可以抑制乳腺癌、结肠癌细胞的增殖与迁移，虽没有影响胰腺癌细胞增殖，却促进其迁移，其对不同肿瘤细胞的影响具有差异性。 [关键词] 艾塞那肽；乳腺癌细胞；结肠癌细胞；胰腺癌细胞 Effect of GLP-1 agonist exenatide on migration and proliferation of tumor cell Zhang Rui， Liu Xiufei， Hui Wang， Wang Xiaocui， Tong Qiang，Zheng Hongting（Department of Endocrinology，Xinqiao Hospital，Third Military Medical University，Chongqing， 40037， China） [Abstract]Objective To observe the effect of exenatide on breast cancer cells, colon cancer cells, pancreatic cancer cells proliferation and migration in vitro. Methods Breast cancer cells MDA-MB-231, colon cancer cells HCT116 and pancreatic cells HS766T were slected as experimental subjects. Each type of cells was divided into control group, metformin group and exenatide group. After administration of metformin and exenatide respevtly, CCK-8 was used for proliferation detection in each group at 72 hours, the migration of tumor cells was observed using Transwell chamber assay at 12 hours. Results Compared with the control group, the proliferation of MDA-MB-231 cells in exenatide group was significantly inhibited (P 0.01), the proliferation of HCT116 cells were inhibited (P 0.05), and the proliferation of HS766T was not effected (P 0.05). Transwell migration assay shows that MDA-MB-231 cell migration was significantly inhibited by exenatide (18.10 ± 5.82, P 0.01), HCT116 cell migration was also inhibited (21.20 ± 5.35, P 0.05), but cell migration in HS766T group was significantly increased by exenatide.