负载转化生长因子β3微球的壳聚糖三维支架的制备.docVIP

负载转化生长因子β3微球的壳聚糖三维支架的制备 蒋 科，熊 雁,余 江，王子明,王爱民 (400042 重庆,第三军医大学大坪医院野战外科研究所骨科) [摘要] 目的 探讨制备负载转化生长因子β3微球的壳聚糖三维支架的可行性。方法 利用冻干法制备壳聚糖三维支架，扫描电镜观察并测定其水结合率及孔隙率。利用乳化交联法制备负载转化生长因子β3的壳聚糖微球，检测负载微球的体外缓释情况及吸水膨胀率。制作负载转化生长因子β3微球的壳聚糖三维支架。结果 壳聚糖三维支架孔隙较为一致，孔隙直径（180.4±35.3）μm，孔隙率（83.2±0.6）%，与水的结合能力为（123±5）%。电镜观察微球表面光滑，分散性好，粒径（28.5±5.1）μm。对壳聚糖微球体外缓慢释放TGF-?3进行连续监测7 d，总释放率为（46.2±0.3）%。负载TGF-?3微球的壳聚糖三维支架观察见微球在支架中分布均匀。结论 负载TGF-?3微球的壳聚糖三维支架的制备技术成熟，理化性质稳定，微球缓释TGF-?3效果理想，可作为理想的组织工程材料。 [关键词] 壳聚糖;转化生长因子β3；微球 [中图法分类号] [文献标志码] A The preparation of controlled-released three-dimensional chitosan scaffold loading with TGF-β3 microspheres Jiang Ke, Xiong Yan, Yu Jiang, Wang Ziming, Wang Aimin (Department of Orthopedics, Institute of Surgery Research, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China) [Abstract] Objective To prepare a three-dimensional chitosan scaffold loading with transforming growth factor-?3 (TGF-?3) microspheres. Methods Three-dimensional chitosan scaffold was prepared by lyophilization, and water bonding capacity and porosity were measured. TGF-?3 loaded chitosan microspheres were prepared by emulsion-crosslinking method. The controlled release of TGF-?3 was monitored for 7 days and the swelling index was measured. At last, the three-dimensional chitosan scaffold loading with TGF-?3 microspheres was prepared. Results SEM images showed the morphology of porous scaffold was uniform, and the mean diameter of interconnected pores was (180.4 ±35.3) μm. The porosity was (83.2±0.6)% and swelling index of microspheres was (123±5)%. The microspheres were spherical and smooth, and the mean diameter of the microspheres was (28.5±5.1) μm. The accumulated release of TGF-β3 reached (46.2±0.3)% within 7 days. Most microspheres were uniform in the scaffold. Conclusion The method for preparing three-dimensional chitosan scaffold loading with TGF-?3 microsphere is feasible, and the sustained release of TGF-?3 is realized. [Key Words] chitosa