谷胱甘肽转移酶基因多态性在微囊藻毒素致人群肝损伤发生中的作用.docVIP

谷胱甘肽转移酶基因多态性在微囊藻毒素致人群肝损伤发生中的作用 李砚，陈济安，赵清，邱志群，许川，曾慧，舒为群（400038 重庆，第三军医大学军事预防医学院环境卫生学教研室） ［摘要］目的 探讨谷胱甘肽转移酶（glutathione S-transferase ,GST）M1（GSTM1）和Tl（GSTT1）基因多态性在微囊藻毒素（microcystins，MCs）致人群肝损伤发生中的作用。方法 在重庆涪陵区采用酶联免疫法检测饮用水和水产品中MCs浓度，并随机在MCs暴露人群中选择肝功异常人群81例，肝功正常人群92例为研究对象进行病例-对照研究，多重PCR方法检测GSTM1和GSTTl基因多态性，采用χ2检验比较各组基因型的差异。结果 饮用水中MCs的浓度连续4年超过国家规定标准，水产品中MCs阳性率为100%；GSTM1缺失频率在肝损伤组和对照组分别为43.2%和47.8%，两组间无显著统计学差异(P=1.00)；GSTT1缺失频率在肝损伤组为60.5%，高于对照组39.1%，两组间有显著统计学差异(P=0.006)；GSTM1和GSTT1共同缺失频率在肝损伤组为29.6%，同样高于对照组中16.3%，两组间有显著统计学差异（p=0.045）。结论 GSTT1缺失可能是在MCs暴露人群肝损伤的易感因素；GSTM1和GSTT1共同缺失将会增加MCs暴露人群发生肝损伤的危险性。 ［关键词］微囊藻毒素；肝损伤；基因多态性；GSTM1；GSTTl Relationship between genetic polymorphisms of glutathione S-transferase and liver damage in microcystins exposure population Yan Li, Ji-an Chen, Qing ZhaoWeiqun Shu（Department of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China） ［Abstract］Objective To study the relationship between the polymorphisms of glutathione S-transferase (GSTT1, GSTM1) and liver damage in microcystins exposure population. Methods We detected the MCs concentration of drinking water and aquatic products in Fuling District of Chongqing by enzyme-linked immunosorbent assay, and selected 81 students with liver damage and 92 healthy controls in MCs exposure population for case-control comparing analysis.The GSTM1 and GSTT1 genotypes were determined by multiplex polymerase chain reaction. The data of genotypes was analyzed by using χ2-test. Results The MCs concentration in drinking water was higher than the national standard for four years, and the positive rates of MCs in aquatic products was 100%. The frequency of GSTM1 null genotype was 43.2% in the case group and 47.8% in controls, the difference was not significant(P=1); The frequency of GSTT1 null genotype in the case group was 60.5% higher than 39.1% in controls, suggesting significant difference between them(p=0.006). The prevacence of GSTM1 and GSTT1