核酸检测技术应用于血液筛查中的检测效能分析.docVIP

核酸检测技术应用于血液筛查中的检测效能分析建议修改，未能概括文中主要内容！?基金项目? 建议修改，未能概括文中主要内容！ ?基金项目? 河北省卫生和计划生育委员会指令性课题(ZL； ?通信作者? 马印图，电话：（0311E-mail163.com 李莉华1，马印图2 （050071 石家庄，河北省血液中心检验科1；解放军白求恩国际和平医院输血科2） ?摘要? 目的 分析比较不同核酸检测模式的核酸检测(nucleic acid technology，NAT)结果及血小板、全血捐献者的核酸检测结果,以评价NAT应用于血液筛查中的的 删除检测效能。方法 2013年1月-2014年10月间所采集的305912份全血和单采血小板标本进行常规ELISA检测,其中的303616份标本进行核酸检测，核酸检测标本分别采用单检模式和混样模式进行核酸检测,分析各项检测结果数据。结果 采用单检模式进行核酸检测，单独NAT不合格率为1.32‰，采用混检模式进行核酸检测，单独NAT不合格率为0.33‰；全血标本的单独NAT不合格率为0.67‰，单采血小板标本的单独NAT不合格率为0.94‰。结论 NAT 应用于血液筛查，其检测效能与检测灵敏度成正比、与献血者献血间隔成反比。对于献血间隔要求较短的单采血小板捐献者实施NAT,其检测效能尤为突出。 删除 ?关键词? 核酸检测（NAT）；检测效能；献血间隔；检测模式 Application Assessment the Screening efficiency of Nucleic Acid Testing in Blood Screening LI Lihua1, MA Yintu2（1Department of Medical Laboratory,Hebei province Blood Center；2Department of Blood Transfusion，Bethune International Peace Hospital of PLA. Shijiazhuang，050071） ?Abstract? Objective To evaluate the screening efficiency of nucleic acid technology (NAT) blood screening through the analysis and comparison of the result of the platelet donors and the whole blood donors. Methods From Jan. 2013 to Oct. 2014,305912 Donors,s blood samples were detected by general ELISA and 303616 samples were detected by NAT. NAT samples were randomly divided into individual nucleic acid technology（ID-NAT） mode and multiplex nucleic acid technology (MP-NAT) mode. The results of two type specimen were analyzed and compared. Results All the specimens were detected, The single disqualification ratio of ID-NAT was 1.32‰ and MP-NAT was 0.33‰.The disqualification ratio between the whole blood donor specimens and the platelet donor specimens was 0.67‰ and 0.94‰.of NAT was 0.067%,while the positive ratio only 0.28% and 0.094% in 27698 platelet donor specimens respectively. Conclusion It was very necessary to carry out the virus NAT on the blood screening. The screening efficiency of NAT in direct proportion to the sensitivity but in inverse proportion t