毫米波辐照对大鼠局部皮肤P物质和组织形态学的影响.docVIP

姚权,等. 毫米波辐照大鼠皮肤损伤效应研究 毫米波辐照对大鼠局部皮肤P物质和组织形态学的影响 张彦文, 姚 权, 余争平，张广斌 （400038重庆，第三军医大学预防医学院劳动卫生学教研室） [摘要] 目的 观察毫米波辐照对SD大鼠局部皮肤P物质和组织形态学的影响。方法：采用40 W/cm2 35 GHz毫米波局部1次辐照SD大鼠30 s、1 min、3 min，测量辐照前后局部皮肤温度的变化，在辐照后不同时相点用HE染色观察局部组织形态学变化，用放射免疫法测定局部皮肤组织中P物质的变化。结果 毫米波辐照大鼠后，大鼠皮肤温度显著升高；皮肤HE染色可见表皮真皮层不同程度组织形态学改变，随辐照时间的增加皮肤损伤加重，可引起胶原致密化和局部变性坏死；大鼠局部皮肤中P物质含量在辐照后第5 min和10 min显著升高，1 h后回复至对照组水平。结论 在本实验条件下，毫米波辐照30 s就可致大鼠皮肤损伤和痛觉反应，这可能与其明显的热效应和P物质释放有关。 [关键词] 毫米波；P物质；热效应；皮肤 [中图法分类号] [文献标志码]A Effects of acute millimeter wave radiation on substance P and histomorphology in rat skin Zhang Yanwen, Yao Quan, Yu Zhengping, Zhang Guangbin （Department of Occupational Health, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China） [Abstract] Objective To observe the effects of acute millimeter wave(MMW) irradiation on substance P and histomorphology in rat skin. Methods The outside skin on SD rat’s back was radiated by 35 GHz MMW (40 W/cm2) for 30s, 1min and 3min respectively. Rat skin temperature was detected before and after irradiation with infrared thermometer. The skin was taken at different timepoints after irradiation for observing histomorphology with HE staining and detecting substance P(SP) level with radioimmunoassay. Results The rat local skin temperature was markedly elevated, and different degrees of tissue rarefaction and congestion and edema were observed in dermal layer of skin after MMW irradiation. And the longer the exposure time was, the more obvious the changes were. Localized dense collagen and necrosis like change in epidermis can be seen eventually. Skin SP level increased significantly at 5 min and 10 min, and it returned to control levels at 1h after MMW irradiation. Conclusions Based on the experiment condition, MMW irradiation could induce the rat skin damage and hyperalgesia, and the effects may be related with markedly elevated skin temperature(thermal effect) and release of SP. [Key words] millim