厄多司坦预处理对大鼠心肌缺血再灌注损伤及心肌细胞凋亡的影响.docVIP

厄多司坦预处理对大鼠心肌缺血-再灌注损伤及心肌细胞凋亡的影响 王洪波，汪 斌，向小勇 （400016 重庆，重庆医科大学附属第一医院胸心外科） [摘要] 目的 探讨厄多司坦预处理对大鼠心肌缺血-再灌注损伤(myocardial ischemia-reperfusion injury，MIRI)的保护作用及对心肌细胞凋亡的影响。方法 雄性SD大鼠30只，完全随机设计法分成伪手术组、缺血-再灌注组及厄多司坦预处理组，每组10只。采用结扎左冠状动脉前降支30min、再灌注120min的方法建立大鼠心肌缺血-再灌注损伤模型。再灌注末，测定血清丙二醛(MDA)含量，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化酶(GSH-Px)、肌酸激酶（CK）、乳酸脱氢酶（LDH）的活性；心肌MDA含量，SOD、CAT、GSH-Px的活性；TUNEL染色检测心肌细胞凋亡。结果 厄多司坦预处理减少再灌注末血清及心肌MDA含量(P＜0.05)，降低血清CK、LDH活性(P＜0.05，P＜0.01），提高血清及心肌SOD、CAT及GSH-Px活性(P＜0.05，P＜0.01），降低心肌细胞凋亡率(P＜0.05）。结论 厄多司坦预处理可减轻在体心脏缺血-再灌注模型大鼠的氧化应激损伤，并可减轻氧自由基诱导的心肌细胞凋亡。 [关键词] 厄多司坦；心肌缺血再灌注损伤；氧自由基损伤；心肌细胞凋亡 [中图法分类号] [文献标志码] A Effect of Erdosteine preconditioning on myocardial ischemia-reperfusion injury and myocardial apoptosis in rats Wang Hongbo，Wang Bin，Xiang Xiaoyong (Department of Cardiothoracic Surgery，First Affiliated Hospital，Chongqing Medical University，Chongqing，400016，China） [Abstract] Objective To investigate the effects of erdosteine preconditioning on myocardial ischemia-reperfusion injury and myocardial apoptosis in rats. Methods 30 male SD rats were randomly divided into three groups：sham operation group,ischemia-reperfusion group and erdosteine preconditioning group. The left anterior descending coronary artery was ligated for 30 minutes and then loosed for 120 minutes to establish the rat model of myocardial ischemia-reperfusion injury .The contents of serum and myocardial maleic dialdehyde (MDA) , the activities of serum and myocardial superoxide dismutase (SOD)、glutathione peroxidase (GSH-PX),catalase (CAT), the activities of serum creatine kinase（CK）and lactate dehydrogenase（LDH）were detected at reperfusion 120 minutes. TUNEL were used to detect the myocardial apoptosis index. Results Erdosteine preconditioning significantly reduced the contents of serum and myocardial MDA(P＜0.05), reduced the activities of serum CK and LDH(P＜0.05,P＜0.01）,enhanced the activities of serum and myocardial SOD,GSH-PX,CAT（P＜0.05,P＜0.01), reduc