不同生物学行为非小细胞肺癌组织中ZNF266基因的差异表达及意义.docVIP

不同生物学行为非小细胞肺癌组织中ZNF266基因的差异表达及意义 赵晓龙 葛明建 黎力 （重庆医科大学附属第一医院胸心外科，重庆400016） 摘要：目的 观察ZNF266（zinc finger protein 266）基因在不同生物学行为非小细胞肺癌(NSCLC)组织中的表达水平及在NSCLC发生、发展及淋巴结转移中的意义。方法 选取30例不伴有淋巴结转移的NSCLC原发灶(A1)及癌旁正常组织标本(A2)和30例伴有淋巴结转移的NSCLC原发灶(B1)及淋巴结转移灶标本(B2)，采用SABC免疫组织化学三步法检测各组标本中ZNF266基因的蛋白表达水平。结果 ZNF266蛋白表达在NSCLC组织中阳性表达率显著高于癌旁正常肺组织(40% vs 7%，?2=9.317，P0.01)；在伴有淋巴结转移的原发灶中的表达明显高于不伴有淋巴结转移的原发灶 (67% vs 40%，?2=4.286，P0.05)；淋巴结转移灶中ZNF蛋白表达水平明显高于原发灶(93% vs 40%，?2=6.667，P0.05)。结论 ZNF266基因可能参与了NSCLC的发生、发展及淋巴结转移。 关键词：非小细胞肺癌；ZNF266;癌转移；免疫组化; Differential expression and significance of ZNF266 in non-small cell lung cancer with differential biological behavior Zhao xiaolong，Ge mingjian,Li li,Department of Thoraco-cardiac Surgery,the First Affiliated Hospital，Chongqing Medical University，Chongqing 400016,China [Abstract] objective ：To detect the expression of zinc finger protein 266(ZNF266) ---------------------------------------------------------------------------- 基金项目：国家自然科学基金（编号[supported by a grant from National Natural Science Foundation of China(No及重庆市自然科学基金（CSCT2008BB5210）[supported by a project of Narural Science Foundation of ChongQing] 通讯作者：葛明建，男，（1970- ），教授，硕士生导师。主要研究方向：肺癌生物学行为及综合治疗；电话：（023E-mail： mingjian_ge@ 作者：赵晓龙，男，（1983-），硕士。E-mail：zxl6036@126.com in non-small cell lung carcinomas (NSCLC) with differential biological behavior and explore the significance of the pathogenesis and development of the disease.Methods: The SABC immuno-histochemistry assay was used to detect the expression of ZNF266 in 30 specimens of primary NSCLC peficancerous tissues and 30 specimens with metastatic tissues in lymph node respectively.Results: The expression level of primary NSCLC tissue was higher than that of the normal peficancerous tissues（40% vs 7%,?2=9.317,P0.01).The expression of primary NSCLC with lymph node metastasis was higher than that without lymph node metastasis(67% vs 40%,?2=4.286,P0.05).The expression level of primary lesion in NSCLC with lymph n