体细胞重编程为多潜能干细胞的研究进展-生命科学.PDF

20 2 Vol. 20, No. 2 2008 4 Chinese Bulletin of Life Sciences Apr., 2008 1004-0374(2008)02-0231-06 ( 150081) (embryonic stem cells,ES cells) ES ES ES DNA Q 81 3 A Studies of somatic cells epigenetic reprogramming to pluripotent stem cells XU Yan-ning, GUAN Na, ZHANG Qing-hua, LEI Lei* (Department of Histology and Embryology, Haerbin Medical University, Haerbin150081, China) Abstract: Nuclear transplantation remains to date the method of choice for creating donor- or patient-matched ES cells, however, the scarce of human oocyte triggered the development of alternative strategies for reprogramming cells to a pluripotent cell, including fusion of differentiated cells with ESCs, incubation of cells in extracts of eggs, ESCs, or pluripotent carcinoma cells and by forced overexpression of pluripotency factors. These approaches elicit at least some reprogramming of nuclear function and accordingly modification patterns of DNA methylation and histone post-translation. Overexpression of defined pluripotency factors in somatic cells leads to transforming these cells to pluripotent ES-cell-like state. Key words: somatic cell; reprogramming; pluripotent stem cell; cell therapy 1997 Dolly [2] ES ES ES 2007-09-18 2007-11-14 [ 1 ] ES (1151hz031) * E-mail lei1086@ 232