低夜温下黄瓜雌性分化相关代谢与蛋白质变化的初步分析-园艺学；蔬洗菜学专业毕业论文.docxVIP

CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ．I Abstract in English．．． ．．．．．．．． ．． ．．． ．．．．．． ． ．．．．．． -．． III 1 Introduction 1 1．1 Purpose and significance 1 1．2 National and abroad literature summarization 。1 1．2．1 Genetic basis of determination in Cucumber 1 1．2．2 The physiological and biochemical basis of differentiation in cucumber and the influence ofenvironment ．2 1．2．3 The transcriptional study of determination in Cucumber ． ． ．．．．．．．5 1．2．4 Advances in proteomics research ．． 6 1．3 Existing problems and prospects ．．．．．．．．．． ． ．．．．．．．．． ．．．．．．．．．． ．．．．．． ．．．-．．6 I．4 Research contents ．．．．．．．．．．．．．． ．． ．．．．．．．．．．．．． ．．．．．．．．．．．．． ．．．．．．．．．．．． ．．7 1．4．1 Main research contents ．．7 1．4．2 Technology routes ． ．．．．．．．．．．．． ． ．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．． ．．．．．．． ．7 2 Materials and methods 8 2．1 Experiment matedals 8 2．2 Plant cultivation and temperature treatments 8 2．3 Sample preparation．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．． ． ．．．．．．．．．．．．．．．．．．．． ． ．．．．．．．．．．．．． ．．8 2．4 Determination ofmetal)olism related indexes ．．8 2．4．1 Determination of sugars ．8 2．4．2 Determination ofhormones and amino acid ．．10 2．4．3 Determination ofrelated enzymes ．．1 1 2．5 Proteome difference analysis．．．．．．． ．． ．．．．．．．．．．．．．．．． ．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．12 2．5．1 Separation ofprotein samples．．．．．．．．． ． ．．．．．．． ．．．．．．．．．．．．． ． ． ．．．12 2．5．2 Determination ofprotein concentration by bradford．．．．．．．．．．．． ． ． ． ． ． ．．．．．．．．．．．．．．．1 3 2．5．3 2-DE two-dimensional gel electrophoresis．．．．．．．．．．．．．．．． ．．．． ． ．．．．．．．．．．．．．14 2．5．4 Gel dyeing．．．．．．．．．．． ． ．．．．．．．．．．．．．．．．．．．．． ． ．．． ．．．．．．．．．．．．．．．16 2．5．5 Gel scanning and image analysis．．．．．．．．．．．．．．．．．． ．．．． ． ． ．．． ．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．． 16 2．5．6 Mass spectrometry analysis．．．．．．．．．．．．．．．． ．．． ． ．． ． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．1 7 2．6 Verification ofprotein differential point in transcription level． ． ．．．． ． ．．． ．．．．．．．．．．．．．17 2．6．1 Extraction ofRNA ．17 2．6．2 Synthesis ofcDNA fi