CD68、CD163阳性巨噬细胞在食管癌组织浸润及对预后的影响.doc

CD68、CD163阳性巨噬细胞在食管癌组织浸润及对预后的影响 解瑞玲1 董伯升2 成媛1 庞慧1李醒亚1(450052 郑州,1郑州大学第一附属医院肿瘤科1,河南郑州,450052；466000河南周口,2周口中心医院肿瘤科2，河南周口 ) ［[摘要］] 目的：探讨CD68 、CD163阳性巨噬细胞在食管癌组织浸润及其对预后的影响。方法：应用免疫组化方法检测CD68、CD163阳性巨噬细胞在食管癌组织的浸润，比较CD68、CD163阳性巨噬细胞浸润密度及对食管癌患者预后的影响。结果：食管癌组织CD68、CD163阳性巨噬细胞浸润密度较癌旁正常组织明显增多, t值分别为21.379和 21.712（P0.05)。 CD68、CD163阳性巨噬细胞浸润密度与患者生存时间均呈负相关性，r分别为-0.281 和-0.344(P＜0.05)。CD68 、CD163阳性巨噬细胞浸润高密度组5年生存率低于低密度组，分别为30.5%vs56.9%， 27.1%vs60.3% (P＜0.05)，但COX风险因素分析CD68阳性巨噬细胞不是独立的预后因素(P＞0.05)，而CD163阳性巨噬细胞是独立的预后因素(P＜0.05)。结论：CD68、CD163阳性巨噬细胞在食管癌组织中具有较高的浸润密度，CD163阳性巨噬细胞浸润密度与患者预后具有密切相关性。 [关键词：] 食管癌；CD68；CD163；巨噬细胞；免疫组化；预后 [中图法分类号] [文献标志码] A The infiltration of CD68、CD163 positive macrophages and its impacts on patients’ prognosis in esophageal cancer Xie Ruiling1，Dong Bosheng2， Cheng Yuan1，Pang hui1， Li Xingya1(1.Department of Oncology，First Affiliated Hospital of Zheng zhou University，Zhengzhou 450052，China；2. Department of Oncology，Zhou kou central Hospital ,Zhou kou 466000,China) [Abstracts] Objective To investigate the infiltration of macrophages expressing the specific marker of CD68 or CD163 in the tissue of esophageal carcinoma and it’s impacts on the patients’ prognosis. Methods Immunohistochemistry was used to detect the infiltration of tumor-associated macrophages in the tissue of esophageal carcinoma and the normal tissue around it. The density of tumor-associated macrophages infiltrated between the esophageal carcinoma and the adjacent normal tissue was compared, while the density of the tumor-associated macrophages was analyzed to find its’impacts on the patients’survival. Results The density of CD68,CD163 positive macrophages in the tissue of esophageal carcinoma is obviously higher than in normal adjacent tissue，t=21.379and t=21.712（P0.05）. The density of CD68,CD163 positive macrophages infiltrated in the tissue of esophageal carcinoma were both negatively correlated with the patients’survival time, r=-0.281 and