籼稻矮化多分蘖突变体gsor23的基因克隆与表达分析.PDFVIP

籼稻矮化多分蘖突变体gsor23的基因克隆与表达分析 王涛1 袁守江2 尹亮2 赵金凤1 万建民1,3 李学勇1,* (1 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程，北京 100081 ；2 山东省水 稻研究所, 山东济南 250100 ；3 南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研 究中心, 江苏南京210095 ；*通讯联系人，E-mail: xueyong.li@ Positional cloning and expression analysis of the gene responsible for the high-tillering dwarf phenotype in the indica rice mutant gsor23 WANG Tao1, YUAN Shou-jiang2, YIN liang2, ZHAO Jin-feng1, WAN Jian-min1,3 and LI Xue-yong1,* (1 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing 100081; 2Shandong Rice Research Institute, Jinan, Shandong 250100; 3 National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu 210095; *Corresponding author, E-mail: xueyong.li@) Abstract: High-tillering dwarf mutant gsor23 was generated from the indica rice variety Indica9 radiatied by γ-ray. Genetic analysis showed that this phenotype was controled by one single recessive gene, which was mapped within a physical distance of 386kb between two InDel markers C1-WT2 and C1-WT4 on the long arm of chromosome 1. There is a known gene D10 within this region, mutations in which caused high-tillering phenotype in rice. Sequence analysis of the D10 allele in gsor23 revealed that the base C at the 404th position in the coding region was deleted, which would cause frameshift mutation after the 134th amino acids. This mutation site and indica background of gsor23 was different from the previously reported japonica mutants d10-1 and d10-2. Therefore, gsor23 is a novel allellic mutant of D10, which encodes the carotenoid-cleaving dio