HPLC法分析玉米须多酚及其抗氧化活性研究.PDFVIP

5 7 Vol. 5 No. 7 2014 7 Journal of Food Safety and Quality Jul. , 2014 HPLC * 李 强, 任 虹 , 王友升, 李 婷 (, , , , 100048) 摘 要: 目的 方法 Folin-Ciocalteu , HPLC ; (ORAC)结果 1.26 mg/g, , , ORAC 469.6 μmol TE/g 结论 , 关键词: ; ; Study on phenolics composition of corn silk and its antioxidant activity in vitro * LI Qiang, REN Hong , WANG You-Sheng, LI Ting (Beijing Key Laboratory of Flavor Chemistry , Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Laboratory forFood Quality and Safety, Food Science College of Beijing Technology Business University, Beijing 100048, China) ABSTRACT: Objective To analyze the phenolics composition of corn silk and its antioxidant activity in vi- tro. Methods Folin-Ciocalteu method was used to evaluate the amounts of total phenolics of corn silk. Phe- nolics composition of corn silk was analyzed by HPLC and the antioxidant activity was evaluated by ORAC method. Results The amount of phenolics in corn silk was 1.26 mg GAE/g. HPLC analysis revealed that 6 phenolic acids (including catechinic acid, chlorogenic, caffeic acid, ferulic acid, phlorizin, and quercetin) were detected. Antioxidant activity assay showed that phenolics of corn silk had an effective antioxidant activity and the average ORAC value was 469.6 μmol TE/g. Conclusion Corn silk is rich in polyphenols with antioxidant activity. KEY