利用家蚕表达合成人表皮生长因子EGF及产物-中国科技论文在线.PDF

2007；33(1) 蚕 业 科 学 CANYE KEXUE 67 利用家蚕表达合成人表皮生长因子(EGF)及产物 对胃粘膜损伤的修复作用 薛仁宇 曹广力 陈 淼 沈卫德 魏育红 贡成良 (苏州大学生命科学学院，苏州 215123) 摘 要 基于家蚕杆状病毒密码子的偏爱性，对人表皮生长因子基因(hEGF)的部分密码子进行修改后合成一个 新的基因，命名为CSEGF。所合成的CSEGF能编码同hEGF完全一致的氨基酸序列，并被重组进家蚕杆状病毒获 得重组Bm—BacCSEGF。重组病毒感染家蚕后，该基因在家蚕中的表达量达28．4 s／mL幼虫血淋~72．33．07 g／mL 蛹血淋巴。将表达CSEGF的蚕蛹经冻干直接制成EGF冻干粉胶囊，经测定冻千粉中EGF的质量比达140 g／g。 动物模型试验显示该冻干粉对酸诱导的急性胃粘膜损伤的SD大鼠有显著的修复作用，对大鼠裸鼠的肾细胞生长 也有明显的促进作用。 关键词 合成人表皮生长因子；基因表达；家蚕；胃粘膜修复 中图分类号 $881．2 文献标识码 A 文章编号 0257—4799(2007)o1—0067—07 Expression of Synthetic h EGF Gene in Bombyx mori and the E仟ect of its Product on Acute Gastric Mucosa Lesion XUE Ren—Yu CAO Guang—Li CHEN Miao SHEN Wei—De WEI Yu—Hong GONG Cheng—Liang (College of Life Science，Soochow University，Suzhou J~ngsu 21 51 23，China) Abstract Based on the codon bias of BmNPV，some codons of human epidermal growth factor(hEGF) gene were modified and named as CSEGF whose deduced amino acid sequence was identically the same as that of hEGF．And the recombinant baculovirus Bm—BacCSEGF with CSEGF was generated．In silkworms．the EGF expression level reached 28．4 Ug per milliliter larva hemolymph and 33．07 IJg per milliliter pupa hemo— lymph，respectively．Further more，the pupae expressed CSEGF were directly used to produce the lyophilized pupa powder whose final concentration of EGF reached 1 40 pg／g Which performed obvious function of plero— sis per OS on acute gastric mucosa lesion induced by acetic acid，and it improved the growth of kidney cell of nude mouse，too Key words Synthesis EGF；Gene expression；Bombyx mori；Gastric mucosa repair 人表皮生长因子(human epidermal growth fac— 53个氨基酸组成的多肽，且含有3个同生物学活性 tor，hEGF)是具有抑制胃酸分泌作用的尿抑胃素，由