不同苦瓜品种的皂苷含量及对_葡萄糖苷酶活性抑制作用的比较_田力东.pdf

2008,41(10):3415-3421 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.10.067 α 1 2 / 510610 510640 Comparison of Saponin Contents of Different Varieties of Momordica charantia L. and Their Inhibition on α-glucosidase Activity 1,2 1 2 1 1 1 TIAN Li-dong , ZHANG Ming-wei , GUO Si-yuan , ZHANG Rui-fen , CHI Jian-wei , WEI Zhen-cheng , 1 1 ZHANG Yan , TANG Xiao-jun 1 ( Bio-Technology Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Food, Ministry of Agriculture, Guangzhou 510610 ; 2College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640) Abstract: ObjectiveTo study the difference in saponin contents of 35 varieties of Momordica charantia L. and their inhibitory effects on α-glucosidase activity. MethodPerchloric acid-vanilin-glacial acetic acid method was used to determine saponin content, while the inhibitory effect of α-glucosidase activity was conducted by colorimetric method. The paired t-test and system clustering procedure were used for this experiment.Result There is a significant difference in contents and their inhibitory effects on α-glucosidase. Activity of saponin extracted from pulp and seed of Momordica charantia L. in different varieties existed. The content ranges of saponin were 1.17%-4.07% and 0.16%-1.54%, with the coefficients of variability bein