中性蛋白酶发酵过程代谢物动态变化的分析.pdfVIP

平的提升提供了重要的理论基础。 关键词：枯草芽胞杆菌；中性蛋白酶；代谢组学；氨基酸 万方数据 ABSTRACT Neutral microbialfermentationis usedin by widely proteinaseproduced soon．A a11d in-d印thstudy indust巧aIld comprehensiVe food，brewing，textile，chemical fementationisof toreducethe costa11dincrease ofthe processgreatsignificance production the ofneutral neutral forcommercialuseis present，theprotease production proteinase．At f．erIllented 1．398 usedinthe B口c玎，姆ofⅥ，hich肋cf，，淞s甜6，f胁ASis、Videly maJlly by indust阱 Selectionof methodiscmcialtothe of an印propriatesamplepretreatment quality metabolomic existsintermsof for Variability s锄plepretreatmenttecluliques analysis．Great inthecurrentliteratureandt11usitisuncenainofasuitable microbialmetabolomics s锄ple this formetabolomicofB口cf，，姗J“6ff凰．In preparationprocedure analysis stud弘the hasbeen aIldsome ofin仃acellularandextracellularme诎01ites a11alyzed dyn锄icChaIlges hadbeen mainworkareasf01lows： usefulconclusions provided．The on the ofmetabolomicof丑J材6ff胁baSedGC-MS，the (1)ToinlproVequality