中药材DNA与重金属快检技术分析.pdfVIP

in detection researchofDNAand metals Rapid technology heavy medicinalmaterials Chinese Abstract isabouttheDNA、PCR and metals study amplificationheavy rapid Objective：T11is detection ofChinesemedicinalmaterials．Inordertomeetthe technology operation that Chinesemedicinal is traditional matedals(TCM)identifyrapid，simple，low system andsensitive a methodtoextract through D1妊，PCR toxicity，highefficiency rapid anddetectionof metalcontent． amplification heavy Method：1．n圮alkalinebufferwasmadeof and1％ lysis soduimnhyoxide，1％PVP TritonX-1 solutionWas thetwo ofheat 00，and髓s—HCI neutralized，throughsteps cracking andneutralizationtoextractDNAfrom ofdifferent methods processedproducts making traditionalChinesemedicine．Onthebasisoftheextraction the of method，the preparation wereusedfor Fried DNA kitandChelex-100method sophorajaponicaproducts universalwereusedfor PCR．2．111e purification，thenprimes amplifying chloroplast CrocussativusL．andits barcodeWas and tofindtheSNPsbetween sequencedanalyzed were for PCRreaction were adulterants．Specificprimersdesignedthe阶咿s，the