03-高效蛋白表系统.pptVIP

高效蛋白质表达系统 1. 原核表达系统 (1)Gram negative (E. coli) (2)Gram positive (B. subtilis) 2. 真核表达系统 (1) Yeast (2) Baculovirus (3) Drosophila (4) Mammalian cells (COS, CHO, BHK etc.) 3. 其它 (1) Cell-free system (2) Protoblast Baculovirus Baculovirus are present in invertebrates primarily insect species. They are not infectious for vertebrates plants. Genome is covalently closed circular double stranded of 134 kbp, due to its small it can accommodate large fragments of foreign DNA. They are divided into two groups on the basis of their structure as Nucleopolyhedroviruses (NPV) and Granuloviruses. These NP