外源物质对板栗淀粉合成及相关酶活性的影响.pdf

第 49 卷 第 4 期 林 业 科 学 Vol. 49 ，No. 4 2 0 1 3 年 4 月 SCIENTIA SILVAE SINICAE Apr ． ，2 0 1 3 doi :10 ．11707 / j ．100 1-7488 ．20 130420 外源物质对板栗淀粉合成 及相关酶活性 的影响 郭素娟 谢 鹏 (北京林业 大 学省 部共 建 森 林 培 育 与保 护 教育 部 重 点实验 室 北京 100083 ) 关键词 : 板 栗 ; 蔗糖 ; 淀粉 ; 酶 活性 ; 调控 中图分类号 : S78 1. 1 文献标识码 : A 文章编号 : 100 1 － 7488 (20 13) 04 － 0 135 － 06 Effects of Exogenous Substance on Starch Synthesis and Related Enzymes Activity in Chinese Chestnut Guo Sujuan Xie Peng (Key Laboratory f or Silviculture and Conservation of M inistry of Education ，Beij ing Forestry University Beij ing 100083 ) Abstract : In this research ，we sprayed 0. 3 % calcium chloride ，5 mmol L － 1 EGTA ，4 % sucrose solution on the surface of leaves to explore the interactions of the total soluble sugar ，sucrose ，and starch content as well as the relevant enzyme activity of Chinese chestnut “yan shan zao feng ”in the fruit establishment stage ． The results indicated that : the sprayed sucrose solution increased soluble sugar ， sucrose ， and starch content ， extended the duration of starch accumulation ，and promoted sucrose synthase ( SS ) ， sucrose phosphate synthase ( SPS ) ， ADPG Pyrophosphorylase (AGPPase) ，soluble starch synthase (SSS) and granule-bound starch synthase (GBSS) activities． Individual fruit weight increased by 5. 15 % ． CaCl2 showed no significant effect on growth and development indexes of Chinese chestnut ，while EGTA treatment reduced the single fruit weight ，disturbed the Chinese chestnut kernel development amd caused formation of E