两株拟盘多毛孢植物内生真菌次级代谢产物与其生物活性研究.pdf

Abstract Abstract Twenty-four compounds (including seven new ones) were isolated and purified from Pestalotiopsis photiniae and Pestalotiopsis zonata by using various chromatographic methods (silica gel column chromatography, preparative thin-layer chromatography, gel chromato -graphy and preparative HPLC). Their structures were elucidated by extensive spectroscopic analysis (1D and 2D-NMR、MS 、IR、UV). Nineteen compounds were obtained from the fermentation broth of the strain Pestalotiopsis photiniae . Four eremophilane-type sesquiterpenoids were isolated from this genus for the first time and named as: dihydroberkleasmin A(1) ，berkleasmin C(2) ，Pestalotiopin A(3) ， Pestalotiopin B(4). Compounds (1) 、(3) 、(4) were new ones. In addition, 9 phthalide derivatives were obtained. Among them, 5-(3-methyl-2-butenyl)-2-hydroxy-3-methoxy- 4-methylbenzoic acid (5), 5-(3-carboxyl-3-methyl-2′E-allyloxy)-3-methoxy-4-methyl phth- alid(6) and 5-(3,3-dimethylallyloxy)-2-methoxy carbonyl-3-methoxy-4-methylbenzoic acid (7) are new compounds. The 9 phthalide derivatives were evaluated for antifungal activities against three plant pathogenic fungi: Fusarium graminearum, Botrytis cinerea, and Phytophthora nicotianae, with MIC values from 3.1 to 50.0 μg/ml (the positive control ketoconazole showed MIC values are 3.1 μg/ml). We tested their antitumor activity, using CAM model. Compound (8) exhibited definite antitumor activity, and the inhibition ratio reached 32.53 %～43.14 % within the concentration range of 10 μg/ml～100 μg/ml. Six compounds were obtained from the fermentation broth of the s