胆酸盐在酸性介质中自聚集作用的共振瑞利散射光谱及其分析应用.pdf

2006 年第64 卷 化 学 学 报 Vol. 64, 2006 第12 期, 1253～1259 ACTA CHIMICA SINICA No. 12, 1253～1259 ·研究论文· 胆酸盐在酸性介质中自聚集作用的共振瑞利散射光谱及其 分析应用研究 南海军 刘忠芳 刘绍璞* (西南大学化学化工学院 重庆 400715) 摘要 研究了牛磺胆酸钠、甘牛胆酸钠、胆酸钠和脱氧胆酸钠等四种胆酸盐在酸性介质中的聚集作用对共振瑞利散射 光谱的影响及其分析应用. 结果表明: 在一定浓度的 HCl, H2SO4 或HNO3 溶液中, 四种胆酸盐均能自聚集形成粒径增 大的聚集体. 该聚集体能导致溶液RRS 显著增强, 并产生了新的RRS 光谱. 不同胆酸盐在同种介质中的反应产物具有 相似的光谱特征, 最大RRS 波长分别位于349 (HNO 介质), 359 (H SO 介质)和369 nm (HCl 介质). 在一定范围内, 胆 3 2 4 酸盐的浓度与散射强度(ΔIRRS)成正比. 对于不同的体系其检出限在 12.0～21.8 ng/mL 之间. 方法灵敏度高, 选择性较 好, 而且十分简便快速. 可用于市售蛇胆川贝液和血清样品中胆酸盐的测定. 关键词 牛磺胆酸钠; 甘牛胆酸钠; 胆酸钠; 脱氧胆酸钠; 共振瑞利散射光谱 Resonance Rayleigh Scattering Spectra of Self-aggregation for Bile Salts in Acidic Media and Their Analytical Application NAN, Hai-Jun LIU, Zhong-Fang LIU, Shao-Pu * (School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715) Abstract In an acid medium of HCl, H SO or HNO , bile salts, such as sodium taurocholate, sodium tau- 2 4 3 roglycocholate, sodium deoxycholate and cow sodium cholate, were able to self-aggregate to form large par- ticles to produce new enhanced resonance Rayleigh scattering (RRS) signals. The RRS peaks were similar in the same medium for the four reaction products. But their spectral characteristics were a little different in the three media. The maximum RRS peak was